miR-551b-3p在胃癌组织中低表达并抑制胃癌细胞系HGC-27的增殖、迁移和侵袭  

miR-551b-3p decreases in gastric cancer tissue and inhibits the proliferation,migration and invasion of gastric cancer cell line HGC-27

作　　者：温馨[1] 翟頔[1] 王芳[1] 余佳[1] 

机构地区：[1]中国医学科学院基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《基础医学与临床》2016年第5期610-614,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金重大研究计划(91440111);国家自然科学基金(31371322)

摘　　要：目的探讨miR-551b-3p在胃癌中的表达变化情况,及其对胃癌细胞功能的影响。方法用real-time PCR的方法检测60例胃癌组织及其对应的癌旁组织中miR-551b-3p的表达量,使用miR-551b-3p模拟物(miR-551b-3p mimic)转染胃癌细胞系HGC-27,CCK-8法检测细胞增殖;划痕愈合实验检测细胞迁移;Transwell法检测细胞侵袭。结果 1)miR-551b-3p在胃癌癌症组织中的表达明显低于癌旁组织(P<0.05)。2)过表达miR-551b-3p mimic可以明显减弱胃癌细胞系HGC-27的增殖、迁移和侵袭能力。结论 miR-551b-3p在胃癌组织中低表达,并且抑制胃癌细胞系HGC-27的增殖、迁移和侵袭,可能与胃癌发生发展密切相关。Objective To investigate the expression and change of miR-551b-3p in gastric cancer and its effect on the function of gastric cancer cells. Methods miR-551b-3p was detected by RT-PCR in 60 cases of gastric cancer samples and control tissues. HGC-27 cell line was infected by miR-551 b-3p mimic,then C CK-8 assay was used to determine the influence of miR-551b-3p on HGC-27 cell proliferation; Wound-healing assay was used to determine cell migration; Transwell assay was used to determine cell invasion. Results 1 ）The expression of miR-551 b-3p in gastric tumor tissues was significantly lower than that of the adjacent tissues （P 〈 0. 05 ）. 2 ） HGC-27 cell proliferation, migration and invasion significantly decreased when the cell was infected by mimic-miR-551 b-3p. Conclusions miR-551 b-3p expresses less in gastric cancer tissues and inhibits gastric cancer line HGC-27 proliferation, migra- tion and invasion, miR-551 b-3p is closely associated with the development of gastric cancer.

关 键 词：miR-551b-3p 胃癌 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]