miR-363-3p在胃癌组织中的表达及其对细胞系HGC-27增殖、迁移与侵袭功能的影响  

Expression of miR-363-3p in gastric cancer tissue and its effect on proliferation,migration and invasion of gastric cancer cell line HGC-27

作　　者：翟頔[1] 温馨[1] 皮静楠 张正一[1] 王芳[1] 余佳[1] 

机构地区：[1]中国医学科学院基础医学研究所北京协和医学院基础学院,北京100005

出　　处：《基础医学与临床》2016年第5期638-643,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金重大研究计划(91440111);国家自然科学基金(31371322)

摘　　要：目的探讨miR-363-3p在胃癌及癌旁样本中的表达差异,并分析其在胃癌细胞系中的功能。方法使用realtime PCR检测59例胃癌组织及对应癌旁组织中miR-363-3p的表达差异;使用miR-363-3p模拟物(miR-363-3pmimic)实现miRNA在胃癌细胞系HGC-27中的过表达,经增殖实验、划痕实验和Transwell实验,检测miR-363-3p对HGC-27细胞功能的影响。结果 miR-363-3p抑制胃癌细胞系HGC-27细胞增殖(P<0.05,P<0.01),抑制胃癌细胞系HGC-27细胞迁移(P<0.001),miR-363-3p对抑制胃癌细胞系细胞的侵袭(P<0.05)。结论 miR-363-3p在胃癌发生中可能起到抑癌作用,并有可能成为对胃癌治疗的一个新靶点。Objective To investigate of the expression of miR-363-3p in gastric cancer and its functional significance in HGC-27 cell line. Methods Real-time PCR analysis was used to detect the relative expression of miR- 363-3p in 59 pairs of gastric cancer tissues and their normal control tissues, miR-363-3p mimics was transfected into HGC-27 cell line for miR-363-3p overexpression. The effects of miR-363-3p overexpression on cell proliferation, migration and invasion were detected by CCK-8, wounding heal and Transwell assays. Results Overexpression of miR-363-3p was seen in gastric cancer cell line （ HGC-27 ）. Proliferation text results showed that miR-363- 3p inhibited cell proliferation. Scratch test results showed that miR-363-3p inhibited cell migration. Transwell test results showed that miR-363-3p inhibited cell invasion. Conclusions miR-363-3p functions as a tumor suppressor miRNA in gastric cancer. Therefore it is a potential target for gastric cancer therapy.

关 键 词：胃癌 HGC-27 miR-363—3p 

分 类 号：R735.2[医药卫生—肿瘤]