魔芋葡甘聚糖/纳米羟基磷灰石/胶原复合材料构建组织工程椎间盘纤维环支架  

Tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan/ nano-hydroxyapatite/collagen composite material

作　　者：庄颖[1,2] 陈希亮[3] 李勇[1] 陈庆华[3] 潘兴华[2] 徐永清[2] 

机构地区：[1]解放军第59医院解放军成都军区脊柱外科微创诊疗中心,云南省开远市661699 [2]解放军成都军区昆明总医院全军骨科中心/干细胞与组织器官工程研究中心,云南省昆明市650032 [3]昆明理工大学材料科学与工程学院,云南省昆明市650093

出　　处：《中国组织工程研究》2016年第16期2412-2417,共6页Chinese Journal of Tissue Engineering Research

基　　金：成都军区医学科学技术研究计划项目(C14013)~~

摘　　要：背景:研究符合天然椎间盘生物学结构与功能的组织工程移植物,对退变椎间盘进行生物学功能重建,已逐步成为治疗椎间盘退变性疾病较理想的解决方案。目的:设计构建一种新型的组织工程纤维环支架材料。方法:以魔芋葡甘聚糖、纳米羟基磷灰石和胶原为原料,依次应用湿法纺丝、化学交联、冷冻干燥等技术构建一种全新的组织工程化纤维环支架,采用X射线衍射分析和傅里叶变换红外光谱仪对其进行定性成分分析,同时对其理化性能、生物力学性能及细胞相容性进行分析。结果与结论:魔芋葡甘聚糖/纳米羟基磷灰石/胶原组织工程椎间盘纤维环支架呈三维立体多孔结构,孔径(425.8±47.3)μm,孔隙率为(73.4±5.6)%,吸水率为(718.6±24.3)%,具有与天然纤维环相似的抗压强度,具有良好的生物相容性,无细胞毒性。结果表明魔芋葡甘聚糖/纳米羟基磷灰石/胶原组织工程椎间盘纤维环支架具有适宜的三维多孔结构、生物相容性、孔隙率、吸水率和生物力学强度。BACKGROUND： Tissue-engineered transplantation technique has become an ideal therapeutic regimen for degenerative disc diseases through reconstituting the biological functions of the degenerated intervertebral discs. OBJECTIVE： To construct a novel tissue-engineered annular fibrosus scaffold. METHODS： Konjac glucomannan, nano-hydroxyapatite and collagen were used to fabricate a new tissue-engineered annular fibrosus scaffold by wet spinning, chemical crosslinking, and freeze drying methods. Afterwards, X-ray diffraction and Fourier transform infrared spectrometer were used to analyze the scaffold qualitative components, physico-chemical property, biomechanical performance and cytocompatibility. RESULTS AND CONCLUSION： The bionic scaffold had a three-dimensional porous structure, with the average pore size of（425.8±47.3） μm, the average porosity of（73.4±5.6）%, and the water absorption of（718.6±24.3）%. In addition, the compressive strength of the scaffold was similar with that of the natural annular fibrosus. More importantly, the scaffold had good biocompatibility without cytotoxicity. These results show that the tissue-engineered annular fibrosus scaffold constructed by konjac glucomannan, nano-hydroxyapatite and collagen has proper three-dimensional porous structure, biocompatibility, porosity, water absorption and biomechanical strength.

关 键 词：硬羟基磷灰石 胶原 组织工程 生物材料 纳米材料 纳米羟基磷灰石 椎间盘 魔芋葡甘聚糖 纤维环 

分 类 号：R318[医药卫生—生物医学工程]