化疗对非小细胞肺癌患者外周血中CD8＋CD28-T细胞表达的影响  

Effect of chemotherapy on the expression of CD8 ＋ CD28 - T cells in peripheral blood of non-small-cell lung cancer patients

作　　者：梁冠中[1] 王艳峰[2] 韩福才[3] 

机构地区：[1]重庆市肿瘤研究所肿瘤内科,404100 [2]山西省肿瘤研究所免疫室 [3]山西省肿瘤研究所呼吸一科

出　　处：《国际肿瘤学杂志》2016年第5期335-339,共5页Journal of International Oncology

摘　　要：目的观察非小细胞肺癌（NSCLC）患者外周血中CD8＋CD28-T细胞的表达水平，研究化疗对CD8＋CD28-T细胞表达的影响及意义。方法采用流式细胞仪检测70例初治NSCLC患者外周血CD8＋CD28-T细胞表达水平，以60例健康体检者作为对照，分析CD8＋CD28-T细胞与肺癌生物学及临床特征间的关系。观察30例NSCLC患者在给予长春瑞滨联合顺铂（NP）、吉西他滨联合顺铂（GP）两方案前后CD8＋CD28-T细胞表达的变化情况。结果NSCLC患者CD8＋CD28-T细胞的表达率为（59．003±15．329）％，明显高于健康对照组（41．036±15．435）％，差异有统计学意义（t=35．904，P=0．001）。CD8＋CD28-T细胞的表达与性别（F=1．374，P=0．697）、病理类型（F=0．779，P=0．509）及临床分期（F=0．070，P=0．933）无关，与年龄（F=15．038，P=0．001）有关。NP组化疗前后的CD8＋CD28-T细胞的表达率分别为（58．793±12．510）％和（55．293±14．637）％，差异有统计学意义（t=2．017，P=0．044）。GP组化疗前后的CD8＋CD28-T细胞的表达率分别为（60．700±16．401）％和（54．127±13．924）％，差异有统计学意义（t=3．007，P=0．009）。结论CD8＋CD28-T细胞在NSCLC患者外周血中高表达，化疗可下调CD8＋CD28-T细胞的表达，为探讨NSCLC化疗联合免疫治疗提供了新的参考。Objective To observe the expression of CD8 ＋ CD28 - T cells in the peripheral blood of non-small-ceil lung cancer （NSCLC） patients, and to investigate the effect of chemotherapy on CD8 ＋ CD28-T cells expression and its clinical significance. Methods Flow cytometry was used to evaluate the level of CD8 ＋ CD28 - T ceils in peripheral blood, of 70 untreated NSCLC patients and 60 healthy controls. The associa- tion between CD8＋ CD28-T cells and the clinical features was analyzed. We also investigated the changes of CD8 ＋CD28 - T cells in 30 NSCLC patients who received chemotherapy by GP （ gemcitabine, cisplatin） and NP （ navelbine, cisplatin）. Results The proportion of CD8 ~ CD28 - T cells in lung cancer group was significantly higher than that iri healthy group （59. 003%±15. 329% vs. 41. 036% ± 15. 435%, t = 35. 904, P = 0.001 ）. No correlation was found between CD8 ＋ CD28 - T cells expression and the gender （ F = 1. 374, P = 0. 697 ）, pathological pattern （ F = 0. 779, P = 0.509 ） and clinical stage （ F = 0.070, P = 0. 933 ）. But CD8 ＋ CD28 - T cells expression was correlated with the age （ F = 15. 038, P = 0.001 ）. The level of CD8 ＋ CD28 - T cells after NP chemotherapy was lower than that before chemotherapy （55. 293%± 14. 637% vs. 58. 793% ± 12. 510% , t =2. 017, P =0. 044）. And the level of CD8 ＋ CD28 - T cells after GP chemotherapy was lower than that before chemotherapy （54. 127% ± 13. 924% vs. 60. 700% ± 16. 401%, t = 3. 007, P = 0. 009）. Conclusion CD8 ＋ CD28 - T cells express highly in NSCLC patients peripheral blood. Chemotherapy down-re- gulates CD8＋ CD28 - T cells expression, which provides a new reference for combination with chemotherapy and immunotherapy in NSCLC patients.

关 键 词：癌 非小细胞肺 药物疗法 CD8阳性T淋巴细胞 

分 类 号：R734.2[医药卫生—肿瘤]