肺癌诊断相关miRNA标志物的筛选和验证  

Screening and validation of miRNAs as biomarkers for diagnosis of lung cancers

作　　者：王闻哲[1] 李伟莉[1] 孟文[2] 马胜林[3] 王孝举[1] 

机构地区：[1]浙江省医学科学院分子医学中心,杭州310013 [2]杭州市第一人民医院胸外科,310006 [3]南京医科大学附属杭州医院,杭州310006

出　　处：《国际流行病学传染病学杂志》2016年第2期86-90,共5页International Journal of Epidemiology and Infectious Disease

基　　金：国家自然科学基金（81172072）;浙江省科技厅国际科技合作专项（2012C24008）;浙江省科技厅公益类项目（2015C33098）;浙江省自然科学基金（R2101405）;杭州市重大科技创新项目（20112313A01）;浙江省卫生科技计划（2016RCA010）;浙江省科技厅协同创新项目（2014F50014）

摘　　要：目的获得肺癌组织和正常肺部组织中差异表达的miRNA用于肺癌的早期诊断。方法首先运用miRNA芯片比较5对来自临床的正常肺部组织和肺癌组织，筛选差异性表达的miRNA，然后用荧光实时定量PCR进行鉴定。最后用ROC曲线分析评价特定miRNA标志物区分肺癌组织与正常肺部组织的能力。结果miR-10b-5p和miR-199a-5p在肺癌组织中的相对表达量（2．76±0．71；2．05±0．38）显著高于肺部正常组织中的相对表达量（1．00±0．17；0．96±0．18），差异有统计学意义（t=2．40、2．56，P〈0．05）。ROC曲线评价miR-10b-5p和miR-199a-5p区分肺癌组织和肺部正常组织的能力，曲线下面积（AUC）分别为0．731和0．672。两个miRNA联合区分肺癌组织和肺部正常组织的AUC为0．773，灵敏度为57．9％，特异性为94．7％。结论miR-10b-5p和miR-199a-5p联合可作为候选肿瘤标志物组合用于早期肺癌诊断。Objective To identify the aberrant miRNAs from cancerous and normal tissues as biomarkers for early diagnosis of lung eaneer. Methods A total of 10 snap-freeze lung tissue specimens with paired normal and cancer were surgically collected and compared by miRNA chips. The aberrant miRNAs were screened and then validated by real-time PCR following the standard protocol. Receiver operating characteristic （ROC） curve analysis was used to assess the performance of miRNAs as biomarkers to distinguish normal and cancerous tissues. Results miR-10b-Sp and miR-199a-Sp were identified with more significant expression levels in patients with lung cancer（2.76±0.71; 2.05± 0.38 ）than normal ones （1.00±0.17; 0.96±0.18 ）, the difference had statistical significance （t=2.40,2.56, P〈0.05 ）. The area under the cm＇ve （AUC） of miR-10b-Sp and miR-199a-Sp were 0.731 and 0.672 respectively in detecting the cancer patients. The multiplexed miRNAs demonstrated a strong discriminative power with an AUC of 0.773 （sensitivity=57.9%, specificity=94.7% ）. Conclusions The combination of miR-10b-Sp and miR-199a-Sp might be potential biomarkers for diagnosis of lung cancer.

关 键 词：微RNAS 诊断标志物 

分 类 号：R734.2[医药卫生—肿瘤]