新疆维吾尔族人群胃癌与维生素D受体基因FokⅠ多态性的相关性  

Association of Fok Ⅰpolymorphism in vitamin D receptor gene with risk of gastric cancer in a Xinjiang Uygur population

作　　者：方法[1] 毕兴[2] 高洁[3] 韩峰[4] 王海江[1] 

机构地区：[1]新疆医科大学附属肿瘤医院胃肠外科,新疆维吾尔自治区乌鲁木齐市830011 [2]新疆医科大学附属肿瘤医院泌尿外科,新疆维吾尔自治区乌鲁木齐市830011 [3]新疆医科大学附属肿瘤医院体检科,新疆维吾尔自治区乌鲁木齐市830011 [4]新疆医科大学附属肿瘤医院急诊科,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《世界华人消化杂志》2016年第11期1734-1740,共7页World Chinese Journal of Digestology

基　　金：新疆医科大学科研创新基金资助项目;No.XJC2013105~~

摘　　要：目的:探讨维生素D受体基因FokⅠ酶切位点多态性与胃癌的相关性.方法:随机选取2013-03/2015-05新疆肿瘤医院就诊的维吾尔族胃癌患者147例(A组)及维吾尔族健康对照者151例(B组),采用多聚酶链反应-限制性酶切片段长度多态性方法测定维生素D受体基因FokⅠ酶切位点多态性,进行两组间比较;将本研究全部胃癌患者经PCR-RFLP测定后按是否带有f基因分为FF组(a组)与Ff+ff组(b组),进行组间D-二聚体测定水平比较.结果:胃癌患者的f等位基因表达频率高于对照组(57.8%vs 47.4%,P<0.05).多因素分析显示:含f等位基因(Ff+ff)的研究对象患胃癌的风险高于不含f等位基因(FF)的研究对象(OR=2.85);含f等位基因组中D-二聚体测定水平较不含f等位基因组明显升高(2.79±1.21 vs 1.09±0.65,P<0.05);随胃癌患者分化严重程度的逐步加重,单纯含F基因(FF)的患者的比例逐步下降(P<0.05).结论:维生素D受体基因FokⅠ酶切位点多态性与维吾尔族胃癌易感性相关联,f等位基因可能是维吾尔族胃癌发生的危险因素之一,而F位点可能是维吾尔族胃癌发生的保护因素.f等位基因可能是调节D-二聚体水平的因素之一,从而影响了维吾尔族胃癌患者的发生或预后.AIM： To investigate whether the FokⅠpolymorphism in the vitamin D receptor gene is associated with risk of gastric cancer.METHODS： A total of 147 Uygur patients with gastric cancer（group A） were randomly selected from March 2013 to May 2015 in the Tumor Hospital of Xinjiang Medical University, and 151 healthy Uygur people（group B） were used as controls. The FokⅠpolymorphism in the vitamin D receptor gene was determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） method, followed by an intergroup comparison. By PCR-RFLP test, all the patients with gastric cancer were divided into an FF group（a） and an Ff ＋ ff group（b）according to whether the patient carried the allele f. The D-dimer level of the two groups was then compared.RESULTS： The frequency of the allele f in gastric cancer patients was higher than that in the control group（57.8% vs 47.4%, P 〈0.05）.Multivariate logistic regression analysis showed that the study subjects carrying the allele f（Ff＋ ff） had a higher gastric cancer risk than those without（FF）（OR = 2.85）. The D-dimer level in the Ff ＋ ff group was significantly elevated compared with the FF group（2.79 ± 1.21 vs 1.09 ±0.65, P 〈0.05）. As the differentiation of gastric cancer worsened, the percentage of patients only carrying the allele F（FF） decreased gradually（P 〈0.05）.CONCLUSION： The FokⅠ polymorphism in the vitamin D receptor gene is associated with susceptibility to gastric cancer. The allele f may be one of the risk factors for gastric cancer,whereas the allele F may be a protective factor.Moreover, the levels of D-dimer in patients with gastric cancer carrying the allele f（Ff,ff） are significantly higher than those only carrying the allele F（FF）, suggesting that the allele f may be a regulator of D-dimer and thus influences the onset or prognosis of gastric cancer in Uygur people.

关 键 词：胃癌 维生素D受体 FokⅠ酶切位点 D-二聚体 f等位基因 

分 类 号：R735.2[医药卫生—肿瘤]