镉胁迫对续断菊与玉米间作体系中植物叶片抗氧化酶活性的影响  

Effects of Cd Stress on Antioxidant Enzymes Activity of Sonchus asper L. Hill. and Zea mays L. in Intercropping System

作　　者：徐佳佳[1] 刘宁宁[1] 秦丽[1] 王吉秀 赵莉[1] 游国柱 

机构地区：[1]云南农业大学资源与环境学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2016年第2期348-355,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金云南联合项目(U1202236);国家大学生创新创业训练计划项目(201310676012);云南省教育厅基金(2010Y340)

摘　　要：为探讨续断菊/玉米间作种植模式对Cd污染的响应机制,通过盆栽实验,研究了不同质量分数的Cd（0,50,100,200 mg/kg）对玉米与续断菊间作体系下植物叶片可溶性蛋白、丙二醛（MDA）含量、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和总抗氧化酶（T-AOC）活性的影响。结果表明：在0和50 mg/kg Cd胁迫条件下,间作玉米和续断菊叶片可溶性蛋白含量降低了8.1%~34.1%,当Cd处理质量分数为100和200 mg/kg时,间作玉米叶片和续断菊叶片可溶性蛋白显著比单作增加了34%~69.1%;间作玉米叶片MDA含量较单作增加了6.1%~64.6%,续断菊叶片MDA含量较单作降低了9.7%~58.7%;在不同Cd质量分数处理下,无论是单作还是间作,玉米和续断菊叶片中SOD、POD、CAT和T-AOC活性均随Cd质量分数的增加而增加,仅Cd质量分数达到200 mg/kg时CAT活性有所下降;同一Cd质量分数处理下,间作玉米叶片SOD、POD（200 mg/kg除外）、CAT和T-AOC活性均较单作降低,分别较低了15.1%~48.3%、2.8%~24.7%、5.2%~42.3%和20.7%~31.9%;间作续断菊叶片T-AOC较单作增加了11.2%~26.4%。总体上,在一定质量分数的Cd胁迫下,玉米和续断菊叶中的MDA含量及抗氧化酶活性均有所提高以维持正常生长,表明抗氧化酶在清除Cd毒害产生的活性氧自由基和提高Cd耐性中有重要作用。Intercropping has shown the potential to phytoremediate heavy metal polluted soils. A pot experiments were carried out to explore the antioxidant responses of Sonchus asper L. Hill and Zea mays L. to Cd pollution under intercropping system. Four cadmium levels（ 0,50,100,200 mg / kg）was designed to study the effects of Cd on the contents of soluble protein and MDA,activities of SOD,POD,CAT and T-AOC in leaves of S. asper and Z. mays. The result showed that： with 0 and 50 mg /kg soil Cd content,the content of soluble protein in S. asper and Z. mays decreased by 8. 1% ~34. 1% under intercropping,and with 100 and 200 mg / kg soil Cd content,the content of soluble pro-tein in S. asper and Z. mays significantly increased by 34% ~ 69. 1%. Compared with monoculture,MDA contents of Z. mays increased by 6. 1% ~ 64. 6% and decreased by 9. 7% ~ 58. 7% in S. asper under intercropping. Under both monoculture and intercropping systems,the SOD,POD,CAT,TAOC activities of S. asper and Z. mays all increased with increasing Cd concentrations. But Cd addition at 200 mg / kg caused CAT activity decrease. Under the same Cd concentration treatment,SOD activity,POD activity（ except 200 mg / kg）,CAT activity and T-AOC activity of Z. mays decreased by15. 1% ~ 48. 3%,2. 8% ~ 24. 7%,5. 2% ~ 42. 3% and 20. 7% ~ 31. 9% in intercropping,compared with monoculture. Under intercropping,T-AOC activity of S. asper increased by 11. 2% ~26. 4%. In general,intercropping could improve antioxidant enzymatic system in Z. mays and S. asper,thus alleviate Cd toxicity under Cd stresses. The enzymatic antioxidation capacity plays important role in Cd tolerance of plant.

关 键 词：镉 间作 续断菊 玉米 抗氧化酶活性 

分 类 号：X173[环境科学与工程—环境科学]