Wnt信号通路抑制剂XAV939对人小细胞肺癌H446增殖及凋亡的影响  

Influence of Wnt inhibitor XAV939 on proliferation and apoptosis of small cell lung cancer H446 cells

作　　者：郭文选[1] 朱瀛谦 沈方臻[1] 王晓妮[1] 潘飞[1] 陈晶[1] 

机构地区：[1]青岛大学医学院附属医院肿瘤科,山东青岛266003

出　　处：《临床荟萃》2016年第5期543-546,共4页Clinical Focus

摘　　要：目的探讨Wnt信号通路抑制剂XAV939对人小细胞肺癌H446细胞增殖和凋亡的影响。方法采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测XAV939、顺铂分别作用及联合用药时H446细胞的生长抑制率,并探索产生最佳细胞生长抑制作用的药物水平,流式细胞仪检测XAV939对H446细胞凋亡情况。结果Wnt信号通路抑制剂XAV939抑制H446细胞生长并呈水平依赖性(P<0.01),顺铂抑制H446细胞生长呈水平和时间依赖性(P<0.01)。两药联合时对细胞生长的抑制作用更明显。流式细胞仪检测结果显示随着XAV939水平增加,H446凋亡细胞比例明显增加(P<0.05)。结论 XAV939通过抑制Wnt信号通路诱导细胞凋亡抑制人小细胞肺癌H446细胞的生长,这为小细胞肺癌的治疗提供辅助治疗手段。Objective To investigate the influence of the Wnt inhibitor XAV939 on the proliferation and apoptosis of small cell lung cancer（SCLC）H446cells.Methods MTT assays was used to detect the growth inhibition rate of cells in each group and to explore the optimized drug concentration.Flow cytometry was used to detect the impact of the XAV939 effects on cell apoptosis.Results The XAV939 inhibited the growth of H446 in a dosedependent manner（P 0.01）,but cisplatin inhibited the growth of H446 in a time and dose-dependent manner（P 0.01）.The combination of both drugs inhibited the growth of H446 more significantly.In addition,the percentage of apoptosis of H446 cells increased with the increased concentration of XAV939（P 0.05）.Conclusion The data indicated that the XAV939 inhibited the growth of SCLC H446 cells by Wnt-induced cell apoptosis,and it may become a helpful treatment tool for SCLC.

关 键 词：小细胞肺癌 细胞凋亡 WNT信号通路 XAV939 

分 类 号：R734.1[医药卫生—肿瘤]