橙皮苷对小鼠抗氧化作用及抗氧化酶基因表达的影响  

Effect of hesperidin on the antioxidant activity and antioxidant enzyme gene expression in mice

作　　者：田美杰[1] 孙英健[1] 官佳懿[1] 沈红[1] 

机构地区：[1]北京农学院动科学院/兽医学(中兽医)北京市重点实验室,北京102206

出　　处：《中国实验动物学报》2016年第2期150-157,共8页Acta Laboratorium Animalis Scientia Sinica

基　　金：北京市教委项目(PXM2013014207000067和KM201410020005);北京农学院科技创新团队科研能力提升工程项目(KCT2014012)

摘　　要：目的探讨橙皮苷(HDN)对机体抗氧化作用的影响。方法采用分光光度法、邻苯三酚自氧化法和Fe2+-邻二氮菲法检测HDN体外清除自由基、抑制线粒体肿胀和红细胞氧化溶血;实验小鼠灌服不同浓度HDN(0、80、160、320 mg/kg)连续12 d,ELISA和分光光度法检测小鼠组织中MDA含量,抗氧化酶(SOD、CAT、GSH-PX)活力,RT-PCR技术分析抗氧化物酶mRNA表达水平。结果与对照组比较,HDN组自由基(·OH、O2-·、DPPH·)清除率明显提高,小鼠红细胞体外氧化溶血和线粒体肿胀显著降低;小鼠组织及血清中MDA含量降低,抗氧化酶SOD、CAT、GSH-PX活力明显高于对照组,组织中抗氧化酶mRNA(SOD、CAT、GSH-PX)表达上调。结论 HDN能够清除自由基,降低自由基引起的细胞氧化损伤,抑制过氧化物生成,上调抗氧化酶基因表达及提高酶活力,呈现良好的抗氧化作用。Objective The aim of this study was to investigate the effects of hesperidin（ HDN） on antioxidant activity in mice. Methods HDN scavenging free radicals was detected by spectrophotometry,inhibition of mitochondrial swelling was detected by pyrogallol autoxidation,and erythrocyte hemolysis was detected by Fe^2 ＋ phenanthroline. The mice were fed with HDN at different concentrations（ 0,80,160,320 mg / kg） by gastric gavage for 12 days. ELISA and spectrophotometric methods were used to assay the amount of MDA in mouse liver and kidney tissues and the activity of antioxidant enzymes（ SOD,CAT,GSH-PX）,and the antioxidant enzyme gene mRNA expression was analyzed by RT-PCR. Results Compared with the control group,the radical（·OH,O2·^-,DPPH·） clearance rate was significantly increased in the HDN groups. There was a significant decrease of oxidative hemolysis of erythrocytes and mitochondrial swelling in vitro.MDA content in the mouse liver and kidney tissues and serum showed a decrease,and the activity of antioxidant enzymes（ SOD,CAT,GSH-PX） in the HDN group was significantly higher than that in the control group. There was an up-regulation of mRNA expression of antioxidant enzyme in mouse liver and kidney tissues. Conclusions The results showed that HDN can eliminate free radicals,reduce cell oxidative damage caused by free radicals,inhibit superoxide production,upregulate antioxidant enzyme gene expression and enhance their enzyme activity,thus showing a good antioxidant effect.

关 键 词：橙皮苷 抗氧化酶 基因表达 

分 类 号：Q95-33[生物学—动物学]