经颈静脉移植神经干细胞治疗脑性瘫痪  

Neural stem cell transplantation for treatment of cerebral palsy

作　　者：娄元俊 刘阳[2] 单海军 曹彩红 介小素 

机构地区：[1]河南中医学院第二附属医院,河南省郑州市450000 [2]河南中医学院研究生处,河南省郑州市450000

出　　处：《中国组织工程研究》2016年第14期2092-2097,共6页Chinese Journal of Tissue Engineering Research

基　　金：河南中医学院苗圃工程项目(MP2011-16)~~

摘　　要：背景:移植入宿主体内的神经干细胞能够存活、增殖、分化为神经元和/或胶质细胞,从而促进宿主缺失功能的部分恢复。目的:观察神经干细胞移植对脑性瘫痪大鼠的治疗作用。方法:将24只大鼠随机分为3组:对照组,模型组和移植组,后两组脑性瘫痪模型建立后1周,移植组大鼠经颈静脉注射1 mL神经干细胞悬液(1×105个),对照组和模型组大鼠经颈静脉注射1 mL生理盐水。移植后3周,测量各组大鼠的趾距、步长和抬高身体摇摆情况,并观察各组大鼠大脑病理组织学变化。结果与结论:(1)模型组大鼠的左前掌趾距和步长明显低于右前掌趾距和步长,差异有显著性意义(P<0.05),移植组和对照组大鼠的趾距、步长均明显高于模型组,差异有显著性意义(P<0.05);(2)模型组抬高身体为不对称摆动,对照组和移植组大鼠为对称摆动;(3)移植组和对照组大鼠的右侧大脑半球面积/左侧大脑半球面积比值明显高于模型组,差异有显著性意义(P<0.05);(4)结果表明,经颈静脉移植神经干细胞可明显改善脑性瘫痪大鼠的病理损伤,并可恢复运动神经行为学功能。BACKGROUND： Transplanted neural stem cells can survive, proliferate and differentiate into neurons and/or glial cells in the host, thereby promoting partial function recovery in the host. OBJECTIVE： To study the therapeutic effects of neural stem cell transplantation on cerebral palsy rats. METHODS： Twenty-four rats were randomly divided into three groups： control group, model group and transplantation group. Animal models of cerebral palsy were made in the latter two groups. One week after modeling, rats in the transplantation group were injected 1 m L stem cell suspension（1×105） via the jugular vein, and rats in the control and model group were given the same volume of normal saline. Toe distance, step length and elevated body swing test in rats were detected, and histopathological changes in the rat brain were observed 3 weeks after transplantation. RESULTS AND CONCLUSION： In the model group, the toe distance and step length of the front left palm were significantly lower than those of the front right palm（P〈 0.05）. Compared with the model group, the toe distance and step length in the transplantation and control groups were significantly increased（P 〈0.05）. In the elevated body swing test, rats in the model group presented with asymmetric swing of the body, but rats in the other two groups exhibited symmetric swing of the body（P 〈0.05）. Additionally, the ratio of right to left hemispheric areas was significantly higher in the transplantation and control groups compared with the model group（P 〈0.05）. In conclusion, neural stem cell transplantation via the jugular vein can improve brain function and restore motor function in rats with cerebral palsy.

关 键 词：神经干细胞 脑性瘫痪 细胞移植 组织工程 干细胞 移植 脑瘫 大鼠 趾距 步长 

分 类 号：R394.2[医药卫生—医学遗传学]