养精胶囊提取液对精原干细胞增殖的影响  

Seminiferous capsule extract effect on the proliferation of spermatogonial stem cells

作　　者：李俊涛[1] 张培海[2] 曲晓伟[3] 李郑生[1] 李松伟[4] 

机构地区：[1]河南省中医学院第一附属医院男科,河南省郑州市450000 [2]四川省中西医结合医院男科,四川省成都市610000 [3]郑州人民医院泌尿外科,河南省郑州市450000 [4]河南省中医学院第一附属医院风湿科,河南省郑州市450000

出　　处：《中国组织工程研究》2016年第14期2104-2109,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(U1204828)~~

摘　　要：背景:临床研究显示补肾填精中药能够促进精原干细胞的增殖和分化。目的:验证养精胶囊提取液对精原干细胞增殖的影响。方法:取雄性小鼠睾丸,分离精原干细胞,用无血清培养液同步化24 h后,加入10,50,100 mg/L养精胶囊提取液24 h,观察对精原干细胞活性、增殖及细胞周期变化。结果与结论:(1)与对照组相比,养精胶囊提取液干预的细胞数量增加、活力增强、S期细胞比例增加,BrdU标记的精原干细胞的数量增加,其中以50 mg/L养精胶囊提取液的效果最为显著;(2)提示养精胶养精胶囊提取液能促进精原干细胞的增殖和促进精原干细胞活力的增加。BACKGROUND： Chinese herbs for kidney nourishment can promote the proliferation and differentiation of spermatogonial stem cells. OBJECTIVE： To verify the effect of seminiferous capsule extract on spermatogonial stem cell proliferation. METHODS： Spermatogonial stem cells were isolated from the testis of male mice and synchronized by serum-free medium followed by an addition of 10, 50, 100 mg/L seminiferous capsule extracts. After 24 hours of culture, viability, proliferation and cell cycle of spermatogonial stem cells were observed. RESULTS AND CONCLUSION： Compared with the control group, seminiferous capsule extracts promoted the cell number, viability and proportion at S stage. The number of Brd U-labeled spermatogonial stem cells was increased significantly after intervention with seminiferous capsule extracts, especially at the concentration of 50 mg/L. These findings indicate that seminiferous capsule extracts can promote the proliferation and viability of spermatogonial stem cells.

关 键 词：精子 细胞增殖 组织工程 干细胞 培养 养精胶囊提取液 精原干细胞 小鼠 细胞培养 无血清培养 MTT法 细胞周期 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]