MMS2和P53基因对结肠癌细胞增殖与凋亡的影响  

Interaction between MMS2 and P53 on proliferation and apoptosis of colon cancer cells

作　　者：马琳园 隋御[1] 马璐[1] 李昕[1] 李元杰[1] 徐方[1] 

机构地区：[1]宁夏医科大学宁夏回族自治区生殖与遗传重点实验室,银川750004

出　　处：《中国免疫学杂志》2016年第4期513-518,共6页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(No.313602501)

摘　　要：目的:探讨MMS2 siRNA与P53 siRNA对人高分化结肠癌细胞(THC-8307)的增殖与凋亡的相互调控作用。方法:以实时定量聚合酶链反应(qRT-PCR)、蛋白印迹法(Western blot)分析检测细胞转染效率,选择沉默效率具有统计学意义的THC-8307细胞作为实验组,同时将未作处理的THC-8307作为空白对照,转染空质粒细胞作为阴性对照。采用qRT-PCR及Western blot分别检测干扰各组目的基因后其两基因相互关系及表达量。采用流式细胞术检测各组细胞的凋亡率,以明确其对结肠癌细胞增殖与凋亡的作用。结果:实验组与对照组相比,分别沉默MMS2基因与P53基因的结肠癌细胞内P53与MMS2的mRNA与蛋白水平显著升高(P<0.05)。沉默MMS2实验组其早期凋亡与晚期凋亡率增加(P<0.05)。结论:MMS2和P53在结肠癌细胞增殖与凋亡方面起反向调控作用。Objective： To explore the regulatory effects of proliferation and apoptosis on THC-8307 by MMS2 siRNA and P53 siRNA. Methods： We experimentally suppressed the MMS2 and P53 expression in human colon cancer cells by the interference RNA technology（ RNAi） as monitored by Real-time qRT-PCR and Western blot. THC-8307 cells that express rate significantly reduced were collected as case group,while using untreated cells as the blank control group,and mock-treated cells as the negative control group. After separately interfering the target genes in each group,test the relationship and expression level of the two genes. Utilizing flow cytometry techniques to test cells proliferation and apoptosis rate of each group. Results： Compared to the control group,colon cancer cells in which MMS2 and P53 were silenced displayed significant increase of P53,MMS2 mRNA and protein levels（ P〈0. 05）. MMS2-depleted cells displayed increase in apoptosis rates,for both early and later stages（ P〈0. 05）. Conclusion： MMS2 and P53 negatively regulate each other in colon cancer cells proliferation and apoptosis.

关 键 词：MMS2 P53 RNA干扰 人结肠癌细胞 细胞凋亡 

分 类 号：R735.35[医药卫生—肿瘤]