胃肠道间质瘤中环氧合酶2及NF-κB的表达  

Expression of cyclooxygenase 2 and nuclear factor-kappa B in gastrointestinal stromal tumors

作　　者：李小红[1] 成元华[1] 郭立新[2] 

机构地区：[1]贵州医科大学病理学教研室,贵阳550004 [2]四川大学华西医院病理科,成都610041

出　　处：《重庆医学》2016年第13期1740-1743,共4页Chongqing medicine

基　　金：贵州省科学技术基金资助项目[黔科合J字(2009)2187号;黔科合J字LKG(2013)27号];贵州省科技计划资助项目[黔科合SY字(2011)3062号];贵阳市科学技术计划[(2009)筑科农合同字第3-020号]

摘　　要：目的探讨胃肠道间质瘤（GISTs）中环氧合酶2（COX2）及核转录因子κB（NF-κB）基因的蛋白表达情况与临床病理特征及预后之间的关系。方法采用免疫组织化学En Vision两步法检测GISTs病例肿瘤组织中COX2及NF-κB的蛋白表达情况，并对患者进行随访。结果115例GISTs中：男61例，女54例，年龄19～80岁，中位年龄55岁，发病高峰年龄56～59岁。肿瘤位于胃75例、肠31例和胃肠外9例；发生于胃者最多见，占65．2％，其次为肠（占27．0％）。梭形细胞为主型95例（82．6％）、上皮样细胞为主型10例（8．7％）和混合细胞型10例（8．7％）。极低危险度组22例、低危险度组33例、中危险度组18例及高危险度组42例。COX2蛋白阳性表达93例，阳性表达率为80．9％；NF-κB蛋白阳性表达94例，阳性表达率为81．7％。COX2蛋白及NF-κB蛋白表达情况在不同危险度分组之间比较差异有统计学意义（P〈0．05），在高危险度组中阳性表达率最高，而在不同性别、年龄、发生部位、组织学类型及是否转移组间比较差异均无统计学意义（P〉0．05）。59例患者获访，随访率51．3％；其中COX2蛋白强阳性表达（＋＋＋）29例，9例转移，1例死亡；NF-κB蛋白强阳性表达（＋＋＋）32例，其中10例转移，1例死亡。结论GIsTs中cox2及NF-κB在的表达与危险度分级相关，是影响患者预后的重要因素之一。Objective To investigate the relation between the expression situation of cyclooxygenase 2（COX2） and nuclear factor-kappa B （NF-κB） gene protein with the clinicopathologic characteristics and prognosis in gastrointestinal stromal tumors （GISTs）. Methods The immunohistochemical EnVision two-step technique was used to assess the expression of COX2 and NF-κB protein in tumor tissue of GISTs. The patients were followed up. Results In 115 cases of GISTs,61 cases were male and 54 cases were female. The age ranged 19-80 years old with the median age of 55 years old,the onset peak age was 56-59 years old. 75 cases of tumor was located in stomach,31 cases in intestine and 9 cases in extragastrointestinal tract. The occurrence in the stomach was most common,accounting for 65.2% ,followed by the intestine （27.0%）. The spindle cell was the predominant type in 95 cases （82.6%） ,epithelioid cell type in 10 cases （8.7%） and 10 cases were mixed cell type（8.7%）. The very low risk group had 22 ca- ses,low risk group had 33 cases,moderate risk group had 18 cases and high risk group had 42 cases. 93 cases positively expressed COX2 with the positive expression rate of 80.9 % ; 94 cases positively expressed NF-κB protein with the positive expression rate of 81. 7%. The difference in the expression of COX2 and NF-κB protein between the different risk groups were statistically significant （P〈0.05） ,the positive expression rate was the highest in the high risk group,but which had no statistically significant difference among different genders, ages,locations, histological types and whether metastasis （P〉0.05）. 59 cases were followed up with the follow-up rate of 51.3 % ;among them 29 cases were strongly positive expression （＋＋＋） of COX2 protein, including 9 cases of metastasis, 1 case of death ; 32 cases of them were strongly positive expression（ ＋＋＋ ） of NF-κB protein, including 10 eases of metastasis and 1 ease and death. Conclusion The COX2 and NF-κB expression in GISTs

关 键 词：胃肠道间质瘤 环氧合酶2 NF-ΚB 免疫组织化学 预后 

分 类 号：R735[医药卫生—肿瘤]