硫化氢对创伤应激引起大鼠急性肝损伤的作用  

The Role of Hydrogen Sulfide in Acute Liver Injury Induced by Traumatic Stress in Rats

作　　者：岑新海[1,2] 张志湘[2] 王涛[1] 王艳莎[1] 季英磊[1,2] 闫骏[1,2] 谷振勇[1,2] 

机构地区：[1]南通大学医学院法医学系,江苏南通226001 [2]苏州大学医学部,江苏苏州215123

出　　处：《法医学杂志》2016年第2期81-85,共5页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81273341);江苏省高校优势学科建设工程项目(PAPD)

摘　　要：目的探讨硫化氢(H_2S)在挤压大鼠后肢致急性肝损伤过程中的作用。方法将大鼠随机分为对照组、挤压组、H_2S供体硫氢化钠(Na HS)+挤压组、H_2S生成抑制剂炔丙基甘氨酸(propargylglycine,PAG)+挤压组。用标准重物挤压大鼠后肢建立急性肝损伤模型,分别于挤压结束后30、120 min处死大鼠。比色法检测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活性,化学法检测大鼠血浆H_2S、肝组织丙二醛(malondialdehyde,MDA)、蛋白质羰基、谷胱甘肽(glutathion,GSH)含量和H_2S生成酶胱硫醚γ-裂解酶(cystathionineγ-lyase,CSE)活性,RT-PCR半定量检测肝组织CSE m RNA的表达。结果挤压大鼠后肢可引起血清AST、ALT活性增高,肝组织MDA、蛋白质羰基含量增加及GSH含量降低,血浆H_2S含量减少,肝组织CSE活性降低、CSE m RNA表达下降。预先给予Na HS可显著减轻、而PAG明显加剧挤压后肢所致上述肝损伤改变。结论 H_2S生成减少参与介导创伤应激引起的大鼠急性肝损伤。Objective To explore the role of hydrogen sulfide （H2S） in acute liver injury induced by crushing hind limbs of rats. Methods The rats were randomly divided into the following groups： control, crushing, H2S donor sodium hydrosulfide （NariS） ＋ crushing, H2S inhibitor propargylglycine （PAG） ＋ crushing group. The acute liver injury model was established by crushing the hind limbs of rats with standard weight. Rats were sacrificed at 30 min and 120 min after the crush. The activities of serum aspartate aminotransferase （AST） and alanine aminotransferase （ALT） were measured by colorimetric method, and the content of HzS in plasma and the contents of malondialdehyde （MDA）, protein carbonyl, glutathione （GSH） in the liver and the activity of H2S generating enzyme （cystathionine γ-lyase, CSE） were determined by chemical method. The expression of CSE mRNA in liver was detected by RT-PCR. Results For crush injury group, the levels of AST and ALT in serum, MDA and protein carbonyl in liver increased. The levels of GSH, CSE, CSE mRNA in liver and H2S in serum decreased. The administration of NariS before limbs crush could attenuate the changes of liver injury, but the pre-treatment with PAG could exacerbate the changes. Conclusion The decrease of H2S production could involve in mediating the acute liver injury induced by traumatic stress in rats.

关 键 词：法医病理学 创伤和损伤 肝 应激 硫化氢 大鼠 

分 类 号：R575[医药卫生—消化系统]