可变数目串联重复序列在河北省鼠疫菌株分型中的应用  

The application of tandem repeat in genetic typing Yersinia pestis of Hebei province

作　　者：王海峰[1] 杨顺林[1] 周松[1] 白雪薇[1] 张懿晖[1] 杨晓燕[1] 牛艳芬[1] 刘合智[1] 杜国义[1] 史献明[1] 

机构地区：[1]河北省鼠疫防治所检验科,河北张家口075000

出　　处：《中国媒介生物学及控制杂志》2016年第2期141-144,共4页Chinese Journal of Vector Biology and Control

基　　金：河北省卫生厅青年科技课题(ZL20140119)~~

摘　　要：目的利用可变数目串联重复序列(VNTR)对河北省鼠疫疫源地菌株进行基因型别研究。方法根据14+12对VNTR设计引物,对116株鼠疫菌进行分析。结果 116株鼠疫菌针对每对VNTR均有不同的重复数目,但是同一对VNTR对所有菌株的VNTR重复数目相同。与CO92和EV对比,河北省鼠疫疫源地菌株显示出不同的扩增结果。结论河北省鼠疫疫源地鼠疫菌只有1种不同于其他疫源地的基因型别,为疫情的追踪溯源和鼠疫菌突变监测提供了依据。Objective To study the genotype of Yersinia pestis in Hebei plague foci by variable number of tandem repeat （VNTR）. Methods Primers were designed according to the confirmed 14＋12 VNTR, to genotype the 116 Y. pestis DNA of Hebei province. Results All of the strains showed one genetype, but they were different from CO92 and EV. Conclusion There is only one genetype of plague, indicating a genetic stability in Y. pestis in Hebei province.

关 键 词：鼠疫菌 可变数目串联重复序列 基因分型 

分 类 号：R254.8[医药卫生—中医内科学]