水产养殖复合微生物制剂中菌群数量的分析  

Analysis of Microbial Flora Quantity of Compound Microbial Preparations for Aquaculture

作　　者：韦成相 雷奇[1] 张福蓉 崔一[2] 郭婉萍 栾春雨 赵晶[1] 权春善[1] 

机构地区：[1]大连民族大学生命科学学院国家民委-教育部生物化工重点实验室,辽宁大连116600 [2]大连工业大学生物工程学院,辽宁大连116034

出　　处：《天津农业科学》2016年第5期41-45,共5页Tianjin Agricultural Sciences

基　　金：中央高校基本科研业务费(DC201502020407)

摘　　要：对3种市售水产养殖复合微生物制剂进行菌群数量检测,利用选择性计数培养基,采用平板计数法和MPN法,分别对3种微生物制剂中的不同菌群进行计数。结果表明,选择性计数培养基能对复合微生物制剂进行有效的菌群计数,初步确定3种水产养殖复合微生物制剂主要由放线菌、芽孢杆菌、乳酸菌、酵母菌、硝化细菌五大类菌群组成,制剂菌群种类和数量与标注有一定偏差,粉剂中有少量霉菌检出。本研究提供的选择性计数培养基及计数方法能够有效对市售复合微生物制剂产品中不同菌群进行计数,为评估同类产品的安全性和有效性奠定基础。Microbial flora quantity detection was carried out aiming at three commercial compound microbial preparations for aquaculture. Colony quantity of different flora in three preparations was counted using plate counting method and MPN method with selective colony count media. The results showed that effective colony count for different microbial flora in compound microbial preparations could be achieved using the selective colony count media. Three preparations mainly consist of five categories including actinomyces,bacilli, lactobacilli, yeasts and nitrifying bacteria. However, for categories of flora and colony quantities of different flora there is a certain deviation between detected results and product description in three preparations. A small amount of molds was also detected in the powder preparation. The selective colony count media and counting methods provided by this study could be used for testing commercial compound microbial preparations for aquaculture. This study laid the foundation for evaluating safety and effectiveness of similar products.

关 键 词：水产养殖 复合微生物制剂 微生物菌群 选择性计数 

分 类 号：S942.3[农业科学—水产养殖]