miR-1290对人脐带间充质干细胞增殖、周期和凋亡的调控作用研究  

Effects of miR-1290 on Proliferation, Cell Cycle and Apoptosis of MSCs

作　　者：周霞[1] 崔丽娜[1] 杨琼[1] 时永全[1] 韩英[1] 

机构地区：[1]第四军医大学西京消化病医院肿瘤生物学国家重点实验室,陕西西安710032

出　　处：《现代生物医学进展》2016年第12期2213-2217,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年基金项目(81400646);国家重大新药创制项目(2014ZX09508002-001;2014ZX09104002-003)

摘　　要：目的:探索microRNA-1290对人脐带间充质干细胞增殖、细胞周期和凋亡的影响。方法:以从胎儿脐带分离的间充质干细胞为基础细胞,构建miR-1290模拟物,进行瞬时转染,通过荧光定量PCR检测转染后miR-1290水平;运用MTT方法观察转染miR-1290后间充质干细胞的增殖情况;通过流式细胞仪染色观察转染后细胞周期和细胞凋亡的变化。结果:荧光定量PCR结果显示,转染后miR-1290表达水平显著升高12.2倍;MTT结果表明,高表达miR-1290相比于阴性对照组(NC组)可以促进间充质干细胞的增殖;流式细胞周期检测结果发现,转染miR-1290后,细胞周期G1期从(85.90±1.91)%缩短至(76.35±2.03)%,而S期和G2期与对照组相比均明显增加,增殖指数(22.75±3.01)相比于对照组(14.10±1.87)也显著升高(P<0.05);凋亡检测结果显示,高表达miR-1290细胞凋亡率(4.9±0.276)%与阴性对照组(8.2±0.891)%相比下降,有统计学意义(P<0.05)。结论:miR-1290可以提高MSC的增殖能力,延长S期和G2期,并一定程度上抑制MSC的凋亡。Objective： To explore the effect of miR-1290 on cell proliferation, cell cycle and apoptosis on human umbilical ling-derived mesenchymal stem cell（MSCs）. Methods： miR-1290 mimics was used to transfected into MSCs derived from human umbilical cord bling. The expression level of miR-1290 after transfection was tested by fluorimetric determination PCR. The proliferation ability was detected by MTT assay. Flow cytometry was applied to assess the cell cycle and apoptosis after transfection into MSCs.Results： RT-PCR results showed that the expression of miR-1290 was upregulated 12.2 fold after transfection. The MTT assay demonstrated that overexpression of miR-1290 could promote the proliferation of MSCs significantly. Compared with control and negative group（NC group）, the period of G1 was decreased from（85.90±1.91）% to（76.35±2.03）%, while S and G2 period of cell cycle were much longer. The proliferation index increased to（23.75±3.01） compared wiith the control group（14.10±1.87）. The result of apoptosis analyzed by flow cytometry showed that the rate of apoptosis of miR-1290（4.9±0.276）%was reduced compared with the negative control（8.2±0.891）%, P〈0.05. Conclusions： miR-1290 could enhance the proliferation ability of MSCs, decrease the period of G1 and extend the S and G2 period, and repress the apoptosis of MSCs.

关 键 词：miR-1290 脐带间充质干细胞 增殖 细胞周期 凋亡 

分 类 号：R575[医药卫生—消化系统] R-33[医药卫生—内科学]