一株联苯基质筛选菌的生理及多氯联苯降解特性  

Physiological and PCB-degrading properties of strain screening form biphenyl substance

作　　者：胡星[1] 杜丽婷[1] 王斐[1] 白晓庆[1] 刘雪岚[1] 杨阳[1] 

机构地区：[1]上海大学环境与化学工程学院,上海200444

出　　处：《上海大学学报（自然科学版）》2016年第2期188-196,共9页Journal of Shanghai University:Natural Science Edition

基　　金：上海市大学生创新创业训练计划资助项目(CXSJ-15-122)

摘　　要：以联苯为唯一的碳源和能源,从未被多氯联苯(polychlorinated biphenyls,PCBs)污染的土壤中筛选、分离出一株生长状况良好的菌株SYC01.分子生物学、生理鉴定结果表明:SYC01为革兰氏阴性、杆状、有鞭毛、能进行趋利避害运动的菌株,属于假单胞菌属;重金属对SYC01的生长多为不利影响,且影响排序为Cu^(2+)Cd^(3+)/Cr^(3+)/Pb^(2+)>Fe^(3+);SYC01对PCB77和PCB52的去除以生物降解为主,降解率分别为34%和68%,是一株能降解高氯代PCBs、高抗毒、降解谱宽的高效降解菌,有原位修复PCBs污染土壤的应用潜力.A strain, SYC01, was isolated from polychlorinated biphenyls （PCBs）- uncontaminated soil using biphenyl as sole carbon and energy source. Molecular and phys- iological assays indicated that SYC01 belonged to Pseudomonas genus, with gram-negative and rods appearance. It could move by its flagellumto avoid being harmed and seek being benefited. Heavy metals had negative impacts on the growth of SYC01 with the effect orders Cu2＋ Cd3＋/Cr3＋/Pb2＋ 〉Fe3＋. The biodegradation rate of PCB77 and PCB52 were 34% and 68%, respectively, suggesting that SYC01 was a high efficient degrader with high chlorinated PCBs degradation ability, high antitoxic capability and broad degradation spectrum. It can be used in in-situ bioremediation of PCBs-contaminated soils.

关 键 词：多氯联苯 生物降解 联苯 菌株SYC01 

分 类 号：X703[环境科学与工程—环境工程]