长链非编码RNA结肠癌相关转录子2在口腔鳞状细胞癌中的表达及临床意义  

Expression of long non-coding RNA colon cancer associated transcript 2 and its clinicopathologicsignificance in oral squamous cell carcinoma

作　　者：欧阳少波[1] 张鹏[1] 王军[2] 黄自坤[3] 廖岚[1] 

机构地区：[1]南昌大学附属口腔医院修复科,330006 [2]南昌大学第二附属医院口腔颌面外科,330006 [3]南昌大学第一附属医院检验科,330006

出　　处：《中华口腔医学杂志》2016年第5期286-291,共6页Chinese Journal of Stomatology

摘　　要：目的分析口腔鳞状细胞癌oralsquamouscellcarcinoma，OSCC）组织特征长链非编码RNA（10ngnon．codingRNA，lncRNA）表达谱，并探讨lncRNA结肠癌相关转录子2（coloncancerassociatedtranscript2，CCAT2）在OSCC中的表达及临床意义。方法采用高通量lncRNA芯片检测3例OSCC患者的癌组织及癌旁正常组织lncRNA表达水平，分析OSCC组织特征性lncRNA表达谱，进一步通过实时定量PCR（real．timequantitativePCR，RT-qPCR）检测86例OSCC及对应的癌旁组织中CCAT2的表达，并分析其与临床病理特征关系。设计合成CCAT2的靶向小干扰RNA（CCAT2-siRNA），脂质体转染舌鳞状细胞癌Tea8113细胞。实验分为阴性对照组和CCAT2-siRNA转染组（实验组），利用CCK-8实验检测CCAT2干预对Tca8113细胞增殖能力的影响。结果OSCC患者癌组织与癌旁对照样本间差异表达的lncRNA共1685个，其中936个表达上调，749个表达下调，差异表达倍数≥10的lncRNA共23个。RT—qPCR验证结果显示，与癌旁组织相比，IncRNACCAT2在OSCC组织中高表达（平均上调5．7倍；P〈0．01）。临床病理相关分析显示，CCAT2表达水平还与OSCC的细胞分化程度及病理分期相关。低分化OSCC组织中CCAT2表达水平明显高于中、高分化OSCC组织（P=0．015）；TNM分期Ⅲ＋Ⅳ期OSCC组织的CCAT2表达水平明显高于Ⅰ＋Ⅱ期标本（P=0．022）。CCK-8实验结果显示，下调舌鳞状细胞癌Tca8113细胞CCAT2表达可明显抑制细胞增殖，转染48、72h实验组A值较对照组显著降低（P〈O．01）。结论多种IncRNA在OSCC患者肿瘤组织中呈异常表达，其中CCAT2在OSCC中的表达上调可能与OSCC的发生发展有关。Objective To investigate the expression of long non-coding RNA（lncRNA） colon cancer associated transcript 2（CCAT2） and its association with clinicopathologic features in oral squamous cell carcinoma（OSCC）. Methods The expression of lncRNA was detected with microarray assay in three samples of OSCC tumor and matched adjacent tissues. The profiles of lncRNAs in OSCC tissues were identified. The CCAT2 expression was evaluated by real-time quantitative PCR（RT-qPCR） in 86 OSCC tumor samples and matched adjacent tissues. The relationship between the expression of CCAT2 and its clinicopathologic features of OSCC was analyzed. Tumor cell proliferation was assessed following siRNA knockdown of CCAT2 by using the CCK-8 kits. Results A total of 1 685 lncRNA expressed in OSCC tumor samples and matched adjacent tissues were identified using microarray assay（P〈0.05）. RT-qPCRshowed that the expression of CCAT2 was significantly higher in OSCC than that in adjacent tissues（P〈 0.01）. High CCAT2 expression was associated with cell differentiation and pathological stage of OSCC. CCAT2 expression in low-differentiated OSCC was significantly higher than that in high-differentiated cancer （P=0.015）. In addition, CCAT2 level in stage Ⅲ＋Ⅳ OSCC was significantly higher than that in stage I /II cancer （P=0.022）. Furthermore, inhibition of CCAT2 expression suppressed the proliferation of human tongue carcinoma Tca8113 ceils. Conclusions Abnormal expression of lncRNA may be involved in the development of OSCC. Up-regulation of CCAT2 expression in tumor tissue might act as an oncogene and promote the development of OSCC.

关 键 词：口腔鳞状细胞癌 长链非编码RNA 结肠癌相关转录子2 

分 类 号：R739.85[医药卫生—肿瘤]