夜磨牙症患者大脑皮质咀嚼区y-氨基丁酸、谷氨酸及N-乙酰天门冬氨酸的磁共振波谱观察  

Measurement of the metabolites in the cortical masticatory area of patients with sleep bruxism： amagnetic resonance spectroscopy study

作　　者：范晓[1] 王继军[2] 刘伟才[1] 

机构地区：[1]同济大学口腔医学院口腔修复学教研室上海牙组织修复与再生工程技术研究中心,上海200072 [2]上海交通大学医学院附属精神卫生中心脑电影像室,200030

出　　处：《中华口腔医学杂志》2016年第5期305-309,共5页Chinese Journal of Stomatology

基　　金：上海市科学技术委员会科研计划（13ZR1444900）

摘　　要：目的采用磁共振波谱（magneticresonancespectroscopy，MRS）技术探讨夜磨牙症患者与健康对照者大脑皮质咀嚼区（corticalmasticatoryarea，CMA）代谢物的差异，探索夜磨牙症患者中枢下颌运动系统的皮质控制部分是否存在异常。方法采用J差分编辑点分辨波谱技术，以水为内源性参照物，测量15例夜磨牙症患者和15名健康对照者大脑双侧CMA内Y-氨基丁酸（gamma—aminobutyricacid，GABA）、谷氨酸及N-乙酰天门冬氨酸（N-acetylaspartate，NAA）水平。用两因素方差分析比较两组受试者双侧CMA各代谢物的差异。结果所有受试者双侧CMA均成功获得编辑波谱。夜磨牙症组右侧和左侧GABA＋、谷氨酸、NAA相对水平分别为（2．45±0．48）×10。和（2．35±0．62）×10^-3、（10．65±1．84）×10^-3和（10．49±2．37）×10^-3、（10．70±3．61）×10^-3和（11．26±4．01）x10^-3。健康对照组右侧和左侧GABA＋、谷氨酸、NAA相对水平分别为（2．63±0．68）×10^-3和（2．65±0．97）x10^-3、（11．19±1．34）×10^-3和（10．58±3．14）x10^-3、（11．82±1．80）x10^-3和（11．95±3．23）x10^-3夜磨牙症组与健康对照组GABA＋（P=0．196）、谷氨酸（P=-O．590）、NAA（P=0．292）差异均无统计学意义；受试者双侧CMA内GABA＋（JP=0．837）、谷氨酸（P=O．510）及NAA（P=O．628）差异也均无统计学意义。结论本组夜磨牙症患者中枢下颌运动系统的皮质控制部分GABA、谷氨酸、NAA与健康对照组无差异。Objective To determine whether there are in vivo differences of metabolites levels in bilateral cortical masticatory area（CMA） of patients with sleep bruxism, compared with healthy controls using proton magnetic resonance spectroseopy（1H- MRS）. Accordingly to explore if cortical control of the central jaw motor system is abnormal in sleep bruxism patients. Methods Fifteen sleep bruxism patients and fifteen age- and gender-matched healthy controls underwent 1H-MRS of bilateral CMA using J-difference edited point-resolved spectroscopy sequenee（MEGA-PRESS） technique. Levels of metabolites were quantified from the ratio of the metabolite integral to the unsuppressed water signal. Differences of levels of Y-aminobutyrie acid（GABA）, glutmate（Glu）and N-acetyl aspartate（NAA） in bilateral CMA between sleep bruxism patients and healthy controls were tested using two-way ANOVA. Results Edited spectra were successfully obtained from the bilateral CMA in all of the participants. Levels of GABA ＋ , glutmate and NAA in right and left CMA in sleep bruxism patients were （2.45±0.48）x 10-3, （2.35±0.62）x 10-3, （10.65± 1.84）x 10-3, （10.49±2.37）x 10-3, （10.70±3.61）x 10-3, and （11.26±4.01）x 10-3 respectively. In contrast, levels ofGABA glutmate and NAA in right and left CMA in healthy controls were （2.63±0.68）x 10 3, （2.65±0.97）x 10 3, （11.19±1.34）x10-3, （10.58±3.14）x10-3, （11.82±1.80）x10-3, and （11.95±3.23）x10-3. There were no differences in levels of GABA ＋ （P=0.196）, Glu（P=0.590）, and NAA（P=0.292） between sleep bruxism patients and healthy controls, nor in inbilateral CMA（GABA ＋ ： P=0.837; Glu： P=0.510; NAA： P=0.628）. Conclusions The results indicate the absence of any alteration of the cortical control of the central jaw motor system in the levels of GABA, Glu and NAA in patients with sleep bruxism.

关 键 词：夜磨牙症 磁共振波谱学 叮氨基丁酸 谷氨酸 

分 类 号：R781[医药卫生—口腔医学]