19个STR座位基因多态性与胃肿瘤的关联性研究  

Association between genetic polymorphism of 19 STR loci and gastric cancer

作　　者：于来水[1] 潘凌子[1] 肖南[1] 梁晓华[1] 刘辉[2] 

机构地区：[1]大连市血液中心,辽宁大连116001 [2]大连医科大学

出　　处：《临床血液学杂志（输血与检验）》2016年第2期267-271,共5页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:探讨19个短串联重复序列(STR)座位(D19S433、D5S818、D21S11、D18S51、D6S1043、D3S1358、D13S317、D7S820、D16S539、CSF1PO、Penta D、vWA、D8S1179、TPOX、Penta E、TH01、D12S391、D2S1338、FGA)基因多态性与胃肿瘤的关联。方法:利用毛细管电泳技术检测38例胃肿瘤患者19个STR座位的基因多态性并进行基因平衡(Hardy-Weinberg)分析,对照组为200例健康个体,通过统计学方法比较两组数据各位点基因频率。结果:实验组19个STR座位基因分布情况符合遗传规律,STR座位检测结果显示:实验组TH01-7、D5S818-15、D3S1358-18、D7S820-9、Penta E-23、D2S1338-19的频率显著高于对照组(P<0.05),D7S820-12、D12S391-17、D2S1338-20的频率显著低于对照组(P<0.05),Penta E座位纯合子的实际频率显著低于理论频率(P<0.05)。结论:TH01-7、D5S818-15、D3S1358-18、D7S820-9、Penta E-23、D2S1338-19 6个基因座附近可能存在胃肿瘤的易感基因,D7S820-12、D12S391-17、D2S1338-20 3个基因座附近可能存在胃肿瘤的抑制基因,Penta E座位的杂合子表现可能增加患胃肿瘤的风险。Objective：To explore the association between gastric cancer and the genetic polymorphism of 19 STR loci（D19S433,D5S818,D21S11,D18S51,D6S1043,D3S1358,D13S317,D7S820,D16S539,CSF1 PO,Penta D,vWA,D8S1179,TPOX,Penta E,TH01,D12S391,D2S1338 and FGA）.Method：The capillary electrophoresis was applied to analyze 19 STR loci in 38 patients with gastric cancer and Hardy-Weinberg balance was tested.Two hundred healthy people were collected as control group.The significant differences in frequency of gene and homozygote were analyzed by statistical methods.Result：The gene distributions of 19 STR in experimental group were consistent with hereditary rules.The frequency of TH01-7,D5S818-15,D3S1358-18,D7S820-9,PentaE-23 and D2S1338-19 in the experimental group were significantly higher than those in the control group（P〈0.05）,while the frequency of D7S820-12,D12S391-17 and D2S1338-20 were obviously lower（P〈0.05）,and the actual frequency of homozygote at the locus PentaE was obviously lower than the theoretical frequency（P〈0.05）.Conclusion：There may be predisposing genes of gastric cancer near the six loci of TH01-7,D5S818-15,D3S1358-18,D7S820-9,PentaE-23 and D2S1338-19,while there may be suppressor gene of gastric cancer near the three loci of D7S820-12,D12S391-17 and D2S1338-20,and the expression of heterozygote at the locus Penta E may increase the risk of gastric cancer.

关 键 词：胃肿瘤 短串联重复序列 多态性 

分 类 号：R735.2[医药卫生—肿瘤]