Wnt/β-catenin信号通路调控下游基因CD44对鼻咽癌侧群细胞的作用和意义  

Effect and its significance of Wnt/p-catenin signaling pathway by controlling tumor marker CD44 on nasopharyngeal carcinoma SP cells

作　　者：熊姗姗 邱前辉[2] 罗小宁[2] 崔勇[2] 苏小妹[2] 许咪咪[2] 

机构地区：[1]深圳市龙岗区第二人民医院耳鼻咽喉科,广东深圳518112 [2]广东省人民医院广东省医学科学院耳鼻咽喉头颈外科,广东广州510080

出　　处：《中国耳鼻咽喉头颈外科》2016年第4期211-215,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：广东省科技计划项目(2012B031800476)

摘　　要：目的探讨Wnt/β-catenin信号通路调控下游基因CD44对鼻咽癌SP细胞的作用和意义。方法通过流式细胞仪从鼻咽癌细胞株CNE-2中分选出SP及CD44^+SP细胞并鉴定,用si RNA阻断Wnt/β-catenin信号通路,了解CNE-2及CD44^+SP细胞在体外的数量及生物学特性改变情况。结果流式细胞仪检测SP细胞在CNE-2细胞中表达率为2.3%,其中36.5%的SP细胞表面表达膜抗原CD44。CD44^+SP细胞的体外增殖能力及对化疗药物的耐受性显著高于其他细胞,分别比较β-Catenin si RNA转染前后的C N E-2及C D 4 4+SP细胞,其细胞增殖能力、克隆能力及对化疗药物的耐受能力均具有统计学差异。结论 Wnt/β-catenin信号通路的异常与鼻咽癌CD44^+SP细胞的生物学意义密切相关,干扰此通路可以改变肿瘤干细胞特性。OBJECTIVE This study aims to explore the molecular mechanism and expression of Wnt/β-catenin signaling pathway and tumor marker CD44 in nasopharyngeal carcinoma cells after transfection withβ-Catenin when the Wnt/β-catenin signaling pathway was blocked.METHODS SP cells and CD44~＋SP cells isolated from the nasopharyngeal carcinoma cell line CNE-2 by flow cytometry were identified.Changes in the number and biological characteristics of CNE-2and CD44~＋SP cells in vitro were investigated after the Wnt/β-catenin signaling pathway was blocked through siRNA.RESULTS SP cells accounted for 2.3%of nasopharyngeal carcinoma CNE-2 cells,andCD44~＋SP cells accounted for 36.5%of the SP cells.CD44~＋SP cells showed significantly higher in vitro proliferation and resistance to chemotherapy（P0.05）.After transfection with β-Catenin siRNA,the proliferation,cloning efficiency,and tolerance to chemotherapeutic drugs of the cells were found statistical differences compared with those before transfection of β-Catenin siRNA.CONCLUSION Wnt/β-catenin signaling pathway abnormalities are closely related to the biological behavior of nasopharyngeal carcinomaCD44~＋SP cells.Interference of this pathway can change the characteristics of nasopharyngeal carcinoma stem cells.

关 键 词：鼻咽肿瘤 肿瘤干细胞 流式细胞术 细胞周期 WNT/Β-CATENIN信号通路 CD44 肿瘤标志物 

分 类 号：R739.63[医药卫生—肿瘤]