miR-187在肺癌组织中的表达及其效应  

Expression of miR-187 in the tissues of lung cancer and its effect

作　　者：孙德彬[1] 蓝秀[1] 

机构地区：[1]丽水市中心医院呼吸内科,浙江丽水323000

出　　处：《温州医科大学学报》2016年第4期278-283,共6页Journal of Wenzhou Medical University

摘　　要：目的:探讨微小RNA(micro RNA,miR)-187在肺癌组织中的表达及其效应。方法:以U6为内参,采用茎环实时荧光定量PCR(RT-q PCR)方法检测32例肺癌及其癌旁正常组织标本miR-187的表达量,并分析其表达与肺癌临床病理特征间的关系。采用miR-187模拟物(mimics)上调A549肺癌细胞内miR-187表达水平,通过噻唑蓝(MTT)法检测细胞活性,通过流式细胞术检测细胞周期。结果:与癌旁正常组织比较,miR-187在肺癌组织中表达显著下调(t=5.236,P<0.001)。临床病理特征相关性分析结果显示,肿瘤组织miR-187的表达与肺癌病理分级及临床分期相关(P<0.05),与年龄、性别、吸烟史、肿块大小、病理类型及淋巴结转移情况未见明显相关性(P>0.05)。采用miR-187 mimics上调A549肺癌细胞内miR-187表达后,细胞增殖明显受到抑制,G0/G1期细胞比例增高,G2/M期细胞比例明显降低。结论:miR-187在肺癌组织中的下调表达可能参与了肺癌的发生发展过程。Objective： To explore the expression of miR-187 in the tissues of lung cancer and its effect. Methods： By normalized to U6, the expressions of miR-187 in 32 lung cancer and matched non-tumor adjacent tissue specimens were examined by stem-loop real-time RT-q PCR method. The relationship between miR-187 expression and clinicopathological characteristics was further analyzed. After transfected with miR-187 mimics, the biological functions of miR-187 were determined by cell proliferation and cell cycle assay. Results： Expression of miR-187 in the tissue of lung cancer was obviously higher than that in non-tumor adjacent tissue specimens（t=5.236, P〈0.0001）. Clinical features correlation analysis showed that the down-expression of miR-187 was correlated with the pathological grading and the clinical staging（P〈0.05）. Functional studies indicated that the overexpression of miR-187 dramatically inhibited the proliferation of A549 cells in vitro and changed the situation of the cell cycle, arrested at G0/G1 phase. Conclusion： Down-expression of miR-187 in the patients of lung cancer may play a key role in the development and progression of lung cancer.

关 键 词：微小RNA miR-187 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]