IL-28B在乙型肝炎相关性肝癌中的表达及其临床意义  

Clinical signifi cance of IL-28B expression in HBV related hepatocellular carcinoma

作　　者：徐峰[1] 于莹[1] 杨永红[1] 刘华[2] 王娟[1] 

机构地区：[1]天津市第三中心医院分院消化科,天津市300250 [2]天津市第三中心医院肝内科,天津市300170

出　　处：《世界华人消化杂志》2016年第12期1812-1818,共7页World Chinese Journal of Digestology

摘　　要：目的:探讨白介素28B(interleukin-28B,IL-28B)在天津汉族人群乙型肝炎相关性肝癌组织中的表达情况,并分析IL-28B的表达与乙型肝炎相关性肝癌临床病理因素之间的关系,探讨其在乙型肝炎相关性肝癌发生发展中的作用及其临床意义.方法:收集96例乙型肝炎相关性肝癌患者与84例乙型肝炎伴发肝脏良性肿瘤患者的血清,采用酶联免疫吸附法检测IL-28B水平、免疫组织化学法检测IL-28B在肝癌组织及肝脏良性肿瘤组织中的表达情况.结果:在乙型肝炎相关性肝癌患者血清中IL-28B的水平明显高于乙型肝炎伴发肝脏良性肿瘤组,免疫组织化学显示IL-28B在乙型肝炎相关性肝癌中的表达阳性率为83.33%,在乙型肝炎伴发肝脏良性肿瘤组织中的表达阳性率为66.66%,两者间的差异具有统计学意义(P<0.05).IL-28B在乙型肝炎相关性肝癌组织中的表达与年龄、性别、HBV DNA、肿瘤分化程度均无相关性(P>0.05),但与甲胎蛋白(α-fetoprotein,AFP)、肿瘤最大径、TNM分期相关(冇2=6.653、6.732、8.642,P<0.05).结论:血清IL-28B的水平对于乙型肝炎相关性肝癌的诊断有到一定价值,其在乙型肝炎相关性肝癌组织中的表达显著升高与肝癌患者的AFP、肿瘤最大径、TNM分期相关,提示IL-28B的表达失调可能参与了乙型肝炎相关性肝癌的发生、发展.AIM：To detect the expression of interleukin-28B（IL-28B） gene in human HBV related hepatocellular carcinoma,and analyze the relationship between IL-28 B expression and clinicopathologic features of HBV related hepatocellular carcinoma.METHODS：Serum samples were collected from96 patients with HBV related hepatocellular carcinoma and 84 patients with HBV related benign hepatic nodules.Serum levels of IL-28 B were measured by enzyme-linked immunosorbant assay（ELISA）.The expression of IL-28 B in HBV related hepatocellular carcinoma tissues was detected by immunohistochemistry.RESULTS：The mean level of serum IL-28 B was significantly higher in the HBV related hepatocellular carcinoma group than in the HBV related benign hepatic nodules group.Immunohistochemistry showed that the positive expression rate of IL-28 B was significantly higher in HBV related hepatocellular carcinoma tissues than in HBV related hepatic benign nodule tissues（83.33% vs 66.66%,P 〈0.05）.IL-28 B expression was not correlated with age,gender,HBV DNA,or tumor differentiation,but it was significantly related to AFP,tumor maximum diameter and clinical TNM stage（X^2= 6.653,6.732,and 8.642,P 〈0.05 for all）.CONCLUSION：The level of IL-28 B has certain value in the diagnosis of HBV related hepatocellular carcinoma.The expression of IL-28 B in HBV related hepatocellular carcinoma tissues signifi cantly increases,which correlates with AFP,tumor maximum diameter and clinical TNM stage.The dysregulated expression of IL-28 B might participate in the genesis and development of HBV related hepatocellular carcinoma.

关 键 词：白介素-28B 乙型肝炎 肝癌 

分 类 号：R735.7[医药卫生—肿瘤] R735.7[医药卫生—临床医学]