肝细胞膜蛋白MRP3、MRP4在大鼠幽门结扎性肝损伤中的表达变化  

Expression of MRP3 and MRP4 in pyloric ligation induced hepatic injury in rats

作　　者：王欢[1] 佟山[2] 红梅[1] 徐艳华[3] 巴图德力根[3] 韩志强[3] 图拉[1] 巴根那[1] 

机构地区：[1]内蒙古民族大学蒙医药学院,内蒙古自治区通辽市028000 [2]内蒙古民族大学附属医院蒙医外科,内蒙古自治区通辽市028000 [3]内蒙古民族大学附属医院蒙药临床研究所,内蒙古自治区通辽市028000

出　　处：《世界华人消化杂志》2016年第12期1853-1859,共7页World Chinese Journal of Digestology

基　　金：国家自然基金资助项目;No.81441105~~

摘　　要：目的:通过实时定量PCR检测(real-time quantitative PCR,qRT-PCR)的方法研究多耐药相关蛋白-3(multidrug resistance-associated protein3,MRP3)、MRP4基因在大鼠幽门结扎性肝损伤中的表达变化.方法:采用幽门结扎法建立大鼠肝损伤模型,测定血清指标、HE染色法在镜下观察病理形态变化、qRT-PCR方法研究MRP3、MRP4在幽门结扎性肝损伤大鼠和正常大鼠肝脏组织中的表达变化.结果:模型组与正常组比较血清谷丙转氨酶(alanine transaminase,ALT)、谷草转氨酶(aspartate transaminase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)、BILD2、SBIL3等均明显增高(P<0.01或P<0.05);肉眼观察模型组大鼠肝脏淤血、腹腔积水;形态学观察肝细胞有明显的脂肪变、水肿、边膜外肝细胞大量坏死、有部分肝细胞凋亡;肝细胞膜蛋白MRP3表达上调至正常组的3.5倍、MRP4表达下调至正常组的0.05倍.结论:幽门结扎引起肝损伤模型的形成,与肝细胞膜蛋白MRP3、MRP4的调节有关.AIM：To detect the expression of drug-resistant related protein（MRP） 3 and MRP4 genes in pyloric ligation induced hepatic injury in rats.METHODS：A rat model of pyloric ligation induced liver injury was fi rst developed.Serum indexes were determined,and HE staining was used to observe pathological changes.Real-time quantitative PCR（qRT-PCR） was used to detect the expression of MRP3 and MRP4 in pyloric ligation induced liver injury.RESULTS：Serum alanine transaminase（ALT）,aspartate transaminase（AST）,alkaline phosphatase（ALP）,BILD2,and SBIL3 were signifi cantly increased in model rats compared with normal rats（P 〈0.01 or P 〈0.05）.Macroscopically,model rats showed liver congestion and peritoneal effusion.Microscopically,obvious hepatocytefatty change,edema,liver cell necrosis,and liver cell apoptosis were noted in model rats.The expression of MRP3 was 3.5 times higher in the model group than in the normal group,while the expression of MRP4 was 0.05 times lower in the model group than in the normal group.CONCLUSION：Pyloric ligation induces liver injury via mechanisms possibly associated with regulating MRP3 and MRP4 expression.

关 键 词：大鼠幽门结扎性肝损伤 病理形态 蛋白表达 

分 类 号：R575[医药卫生—消化系统]