辣椒碱对3T3-L1前脂肪细胞葡萄糖摄取的影响  

Effects of Capsaicin on Glucose Uptake in 3T3-L1 Preadipocytes

作　　者：李倩[1] 程宣[2] 车冠达 李双双[3] 黄昀[1] 刘轩[1] 邹先伟[1] 陈本科[1] 唐劲天[1] 

机构地区：[1]清华大学工程物理系粒子技术与辐射成像教育部重点实验室,北京100084 [2]北华大学化学与生物学院,吉林吉林132013 [3]河北北方学院药学院,河北张家口075000

出　　处：《现代生物医学进展》2016年第14期2601-2603,2650,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨辣椒碱对3T3-L1前脂肪细胞葡萄糖摄取的影响。方法:不同浓度的辣椒碱作用于3T3-L1前脂肪细胞,采用MTT测定细胞活性,GLU Test试剂盒法测定葡萄糖摄取,Western Blot法检测葡萄糖转运蛋白1(GLUT-1)表达的变化。结果:25μM辣椒碱作用72 h和50μM、100μM辣椒碱作用48 h、72 h,可显著抑制3T3-L1细胞增殖,6.25、12.5、25μM辣椒碱作用可显著促进3T3-L1细胞的葡萄糖摄入,Western Blot结果显示辣椒碱能够显著增加GLUT1蛋白表达量,差异均具有统计学意义(P<0.05)。结论:低剂量辣椒碱具有降糖作用,其作用机制可能与增加GLUT-1蛋白表达有关。Objective： To evaluate the effect andpotential mechanism of capsaicin on the glucose uptake in 3T3-L1 preadipocytes in vitro. Methods： 3T3-L1 preadipocytes cells were exposed to different concentrations capsaicin for 24 h, 48 h and 72 h. MTT assay was used to detect their effect on the cell vitality. Glucose metabolism was determined by GLU Test Kits. The GLUT-1 protein was demonstrated by Western Blot. Rosttlts： Treatment of capsaicin （25 μM, 50 μM, 100 μM） showed significant effect on cell viability. Capsaicin （6.25, 12.5, 25 μM） could improve glucose uptake in 3T3-L1 cells. Western Blot results showed that the glucose transporter 1 （GLUT-l） protein expressions were significantly increased in treatment groups than control. Conelusiom： The effect of capsaicin on glucose metabolism might be associated with promoting the GLUT-1 protein expression.

关 键 词：辣椒碱 3T3-L1细胞 葡萄糖摄取 葡萄糖转运蛋白-1 

分 类 号：R-33[医药卫生] R285.5