Tfh细胞在NOD小鼠糖尿病发病过程中的作用机制的研究  

The Role of Tfh in Diabetes of NOD Mice

作　　者：关蛟 张正筠[1] 周尊强[1] 佟大年[1] 周光文[1] 

机构地区：[1]上海交通大学附属第六人民医院普外科,上海200233

出　　处：《现代生物医学进展》2016年第14期2604-2607,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81170721);上海市科委医学引导项目(14411960700);上海市科委浦江人才项目(14PJ1407300)

摘　　要：目的:研究滤泡辅助性T细胞(Follicular Helper T cell,Tfh)在非肥胖性糖尿病小鼠(Non-obese Diabetic mice,NOD)发病过程中的作用机制。方法:实验动物NOD小鼠按血糖值分为胰岛炎组(血糖浓度≤9 mmol/L)及糖尿病组(血糖浓度≥20 mmol/L)。ELISA法检测各组中糖尿病自身抗体谷氨酸脱羧酶抗体(65-kda glutamate decarboxylase antibody,GAD65Ab)、抗胰岛素自身抗体(Insulin autoantibody,IAA)表达水平,Western blot检测B细胞型淋巴瘤6蛋白(B-cell lymphoma 6 protein,Bcl-6)及可诱导共刺激分子(Inducible costimulatory molecule,ICOS)表达,流式细胞仪检测各组外周血及脾脏Tfh细胞水平。结果:糖尿病组NOD鼠自身抗体GAD65Ab(1.21±0.23 nmol/L)、IAA(0.96±0.12 nmol/L)浓度较胰岛炎组(0.32±0.09 nmol/L,0.25±0.06 nmol/L)均有明显升高;糖尿病组NOD鼠Bcl-6及ICOS表达较胰岛炎组NOD鼠有明显升高,外周血和脾脏Tfh细胞水平糖尿病组NOD鼠(24.55%)较胰岛炎组NOD鼠(4.27%)升高明显。结论:NOD小鼠自发糖尿病与自身抗体浓度升高相关,Tfh细胞可能参与NOD鼠糖尿病发生及发展过程。Objective： To research the function mechanism of Follicular Helper T cell （Tfh） in Non-obese Diabetic mice （NOD）. Methods： The NOD mice were divided into two groups according to their blood glucose level （Glu）： the insulitis group （Glu≤ 9 mmol/L） and the diabetic group （Glu≥ 20 mmol/L）. ELISA was used to test the level of diabetes autoantibody 65-kda glutamate decarboxylase antibody （GAD65Ab） and Insulin autoantibody （IAA）. B-cell lymphoma 6 protein （Bcl-6） and Inducible costimulatory molecule （ICOS） were tested by Western blot. Tfh cell count was tested by Fluorescence activated cell sorting （FACS）. Results： The level of GAD65Ab and IAA were much higher in diabetic group than that in insulitis group; Bcl-6 and ICOS were higher expressed indiabetic group than that in insulitis group; Tfla cell count were much higher in diabetic group than that in insulitis group. Conclusions： The autoantibody contributed to the diabetes progression and Tfh cell may participate in the pathogenesis of diabetes in NOD mice.

关 键 词：TFH 糖尿病 NOD 

分 类 号：R-33[医药卫生] R587.1