吲哚胺2,3双加氧酶对人绒毛膜癌JEG-3细胞增殖及迁移能力的影响  

Effect of indoleamine 2,3-dioxygenase on proliferation and migration in JEG-3 cells

作　　者：宗姗姗 赵鑫[1] 罗成凤[1] 李春青[1] 王凯[1] 周倩[1] 

机构地区：[1]同济大学附属第一妇婴保健院妇产科,上海200040

出　　处：《同济大学学报（医学版）》2016年第2期31-34,共4页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金(81270703);上海市科学技术委员会项目(14140900203)

摘　　要：目的观察吲哚胺2,3双加氧酶(indoleamine 2,3-dioxygenase,IDO)对人绒毛膜癌JEG-3细胞体外增殖、迁移能力的影响。方法通过IDO抑制剂(1-L-MT)及短发夹RNA基因干扰抑制IDO表达,利用MTS增殖实验、Transwell迁移试验检测对细胞增殖和迁移能力的影响。结果基因敲减能明显降低IDO表达水平。1-L-MT和基因敲减的JEG-3细胞与对照组相比,细胞的增殖和迁移能力明显下降。结论抑制IDO能够降低绒癌细胞系JEG-3细胞的增殖和迁移能力。Objective To investigate the effect of indoleamine 2, 3-dioxygenase （IDO） on proliferation and migration of human choriocarcinama JEG-3 cells. Methods JEG-3 cell function was studied by IDO inhibitor （1-L-MT） or shRNA. The proliferation and migration of JEG-3 cells were determined by MTT assay and Transwell system, respectively. Results shRNA knockdown significantly decreased IDO expression. 1-L-MT and shRNA knockdown of IDO decreased the proliferation and migration of JEG-3 cells. Conclusion The inhibitory of IDO can suppress the proliferation and migration of trophoblast cells in vitro.

关 键 词：绒毛膜肿瘤 吲哚胺2 3双加氧酶 JEG-3 细胞增殖 细胞迁移 

分 类 号：R737.33[医药卫生—肿瘤]