小鼠酒精性肝损伤过程中肝糖原的表达变化  

Expression changes of hepatic glycogen in mice with alcohol-induced liver injury

作　　者：尚付梅 李三强[1] 卢华杰[1] 姜雅坤 乔新杰[1] 白淼水 霍续磊 李小苹[1] 李思源[1] 李英娇[1] 

机构地区：[1]河南科技大学医学院肝损伤与修复分子医学重点实验室,河南洛阳4710030

出　　处：《中国临床药理学杂志》2016年第9期818-820,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81201558);河南省高校科技创新人才支持计划基金资助项目(13HASTIT025);河南科技大学大学生研究训练计划(SRTP)基金资助项目(2015113);河南科技大学创新团队基金资助项目(2015XTD003)

摘　　要：目的研究肝糖原在小鼠酒精性肝损伤过程中的动态变化及意义。方法 60只雄性小鼠随机分为2组:正常组(n=10),模型组(n=50)。正常组小鼠直接处死取肝;模型组小鼠每天给予56度红星二锅头(15 m L·kg^(-1)),连续灌胃5周,建立酒精性肝损伤模型。于建模第1,2,3,4,5周,各处死10只,取肝组织。用糖原过碘酸雪夫氏(PAS)染色,检测各组肝组织切片中肝糖原的表达情况即平均光密度值(MOD)。结果与正常组的MOD值(0.22±0.04)相比,模型组第1周(0.19±0.06 vs 0.22±0.04,P<0.01),第2周(0.17±0.05 vs0.22±0.04,P<0.01),第3周(0.14±0.02 vs 0.22±0.04,P<0.01),可见肝糖原含量依次下降,至第3周(0.14±0.02)肝糖原含量最低;而模型组第4周(0.17±0.07 vs 0.14±0.02,P<0.01),第5周(0.19±0.03 vs 0.17±0.07,P<0.05)肝糖原含量呈上升趋势。结论小鼠酒精性肝损伤过程中,肝糖原的变化规律为先减少后增加。Objective To investigate the expression changes and significance of hepatic glycogen during liver injury of mice induced by alcohol.Methods Sixty clean and healthy male mice were randomly divided into two groups： normal group（ n = 10） and model group（ n = 50）. All animals in normal group were executed to obtain the liver tissue. The model mice were administered with alcohol of 56 degrees（ 15 mL·kg^-1） for five weeks to induceliver injury,ten of which were given to execute after feeding 1,2,3,4,5 weeks,respectively. The expression levels of hepatic glycogen in all groups were detected by periodic acid- schiff（ PAS） staining（ MOD）. Results Compared to the normal group [MOD,（ 0. 22 ±0. 04） ],the levels of hepatic glycogen were decreased gradually in the model group at 1- week（0. 19 ± 0. 06）,2- week（ 0. 17 ± 0. 05）,at 3- week（ 0. 14 ± 0. 02; all P〈0. 01） was the lowest. Compared to the mice in model group at 3- week（ 0. 14 ± 0. 02）,the hepatic glycogen contents were increased significantly at 4- week（ 0. 17 ± 0. 07; P〈0. 01） and 5- week（ 0. 19 ± 0. 03; P〈0. 05）. Conclusion In the process of alcohol- induced liver injury in mice,the levels of hepatic glycogen were decreased gradually at the early stage,and then increased in the late.

关 键 词：酒精性肝损伤 肝糖原 过碘酸雪夫氏染色 

分 类 号：R575.5[医药卫生—消化系统] R363.21[医药卫生—内科学]