亚洲棉和雷蒙德氏棉MATE基因家族生物信息学及其同源基因在陆地棉中的表达分析  

Bioinformatic Analysis of the Multidrug and Toxic Compound Extrusion Gene Family in Gossypium arboreum and Gossypium raimondii, and Expression of Orthologs in Gossypium hirsutum

作　　者：沈知临 许磊[1] 陈文[1] 吴楠[1] 蔡永萍[1] 林毅[1] 高俊山[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《棉花学报》2016年第3期215-226,共12页Cotton Science

基　　金：安徽省"115"产业创新团队(皖人才办[2011]2号);安徽省生物学重点学科(2014SWQJ009)

摘　　要：多药和有毒化合物排出(MATE)蛋白家族是1个次级转运蛋白家族。为了更好地了解亚洲棉和雷蒙德氏棉中MATE蛋白的种类和数量,利用2种二倍体棉花基因组的氨基酸和c DNA数据库对MATE基因家族进行筛选,并分析亚洲棉和雷蒙德氏棉全基因组中MATE基因的种类和进化关系。结果显示,在亚洲棉基因组中初步鉴定了34个MATE基因,而在雷蒙德氏棉中筛选出42个MATE基因。基因结构和系统进化的比较分析表明,进化关系近的MATE基因在结构上基本是一致的。同时对陆地棉中克隆的Gh TT12及与其同一族的部分MATE基因进行荧光定量分析,推测其所在同一族的MATE基因功能可能与转运原花青素有关。这些结果为进一步深入分析棉花MATE基因的功能以及在原花青素转运中的作用提供了理论基础。The multidrug and toxic compound extrusion（MATE） protein belongs to a secondary transporter family. To better understand the variety and quantity of MATE protein in Gossypium arboreum and G. raimondii, amino acid sequences and cDNA databases from two diploid cotton genomes were used to screen the MATE gene family, and to analyze species and evolutionary relationships of MATE genes. Thirty-four MATE genes were preliminarily identified in the genome of G. arboreum, and 42 in the G. raimondii genome. Comparative analyses of gene structure and phyletic evolution revealed the evolutionary relationship between MATE genes is consistent with structure. Gh TT12 from upland cotton, and other MATE genes from the same family, were analyzed by qRT-PCR. Results suggested the function of MATE gene members was likely related to the transfer of proanthocyanidin. These results provide a theoretical basis for further analysis of MATE gene function and its role in the transfer of proanthocyanidin processes in cotton.

关 键 词：亚洲棉 雷蒙德氏棉 陆地棉 MATE基因家族 基因结构 系统进化分析 荧光定量PCR 

分 类 号：S562[农业科学—作物学]