巴西橡胶树胶乳和悬浮细胞中MVA和MEP代谢途径基因的表达分析  

Expression analysis of MVA and MEP metabolic pathways genes in latex and suspension cells of Hevea brasiliensis

作　　者：邓小敏[1] 吴绍华[1] 戴雪梅[1] 田维敏[1] 

机构地区：[1]中国热带农业科学院橡胶研究所/农业部橡胶树生物学重点开放实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室,海南儋州571737

出　　处：《广西植物》2016年第4期449-455,396,共8页Guihaia

基　　金：国家自然科学基金(31170642);中国热带农业科学院橡胶研究所基本科研业务费(1630022015010)

摘　　要：MVA和MEP代谢途径是植物类异戊二烯代谢途径的两条重要次生代谢途径。该研究利用荧光定量PCR技术,分析了橡胶树胶乳和橡胶树花药愈伤组织来源的悬浮细胞中MVA代谢途径和MEP代谢途径中关键基因的表达水平,同时分析了茉莉酸的结构类似物冠菌素(coronatine,COR)对悬浮细胞中Hb AACT3,Hb HMGR4,Hb HMGR5,Hb DXS2,Hb DXR和Hb SQS1基因表达的调节作用。结果表明:在MVA代谢途径中,基因Hb AACT1,Hb AACT2,Hb HMGS1,Hb HMGS2,Hb HMGR1,Hb HMGR3,Hb MVK,Hb PMK,Hb MVD1,Hb MVD2和IPP下游代谢基因Hb IPPI1和Hb FDPS1在胶乳中的表达量要相对高于其在悬浮细胞中的表达量,然而橡胶树悬浮细胞中MEP代谢途径基因Hb DXS1,Hb DXS2,Hb DXR,Hb CMS1,Hb CMS2,Hb CMK,Hb MCS1,Hb MCS2,Hb HDS,Hb HDR和鲨烯合酶基因Hb SQS1的表达水平要相对高于胶乳。而且COR能不同程度地上调Hb HMGR5,Hb HMGR4,Hb SQS1,Hb DXS2和Hb DXR基因的表达水平。该研究结果为探索利用橡胶树悬浮细胞体系研究次生代谢合成调控以及生产活性次生代谢产物奠定了基础。The MVA and MEP metabolic pathways are two important plant isoprenoid metabolic pathways in plants. The expression of genes respectively in MVA and MEP secondary metabolic pathways were analyzed in the latex and suspension cells from anther-derived callus of Hevea brasiliensis by using qRT-PCR technology. In addition,expression changes of Hb AACT3,Hb HMGR4,Hb HMGR5,Hb DXS2,Hb DXR and Hb SQS1 genes were further analyzed in the suspension cells under COR treatment. The results demonstrated that expressions of Hb AACT1,Hb AACT2,Hb HMGS1,Hb HMGS2,HbHMGR1,Hb HMGR3,Hb MVK,Hb PMK,Hb MVD1,Hb MVD2 in MVA metabolic pathway and Hb IPPI1 and Hb FDPS1 genes involved in IPP utilization were relatively higher in latex than that in suspension cells,while Hb DXS1,Hb DXS2,Hb DXR,Hb CMS1,Hb CMS2,Hb CMK,Hb MCS1,Hb MCS2,Hb HDS and Hb HDR in MEP metabolic pathway and Hb SQS1 were relatively higher in suspension cells than that in latex. Moreover,Hb HMGR5,Hb HMGR4,Hb SQS1,Hb DXS2 and Hb DXR genes were induced highly or to some degree in suspension cells by COR application. This study lays a foundation for further utilization of suspension cells to analyze secondary metabolism regulation as well as to produce bioactive compounds from anther-derived callus of Hevea brasiliensis in the future.

关 键 词：巴西橡胶树 甲羟戊酸 脱氧木酮糖-5-磷酸 冠菌素 鲨烯合酶基因 

分 类 号：Q945.4[生物学—植物学] Q786