DOPEY2在TMPRSS2-ERG基因融合阴性前列腺癌中的表达及临床意义  

The significance of DOPEY2 expression in TMPRSS2-ERG gene fusion-negative prostate cancer

作　　者：罗子寰[1] 陆敏华[1] 黄群雄 张凌霄[1] 陈晓波[1] 高新[1] 李辽源[1] 

机构地区：[1]中山大学附属第三医院泌尿外科,广州510630

出　　处：《中华腔镜泌尿外科杂志（电子版）》2016年第2期52-56,共5页Chinese Journal of Endourology(Electronic Edition)

基　　金：国家自然基金面上项目(81472383);广东省自然科学基金资助面上项目(S2013030011540)

摘　　要：目的探讨DOPEY2在TMPRSS2-ERG基因融合阴性前列腺癌中的表达及其临床意义。方法本研究通过RT-PCR和Western Blot技术检测前列腺癌细胞株VCa P和PC-3中ERG和DOPEY2的表达情况,同时,采用免疫组化技术检测46例前列腺癌根治术后的石蜡标本中ERG和DOPEY2的表达情况,分析两者的表达以及与临床病理特征之间的关系。结果 DOPEY2在TMPRSS2-ERG基因融合阳性前列腺癌细胞株VCa P中的m RNA和蛋白表达水平,均比TMPRSS2-ERG基因融合阴性前列腺癌细胞株PC-3低,且差异均有统计学意义(P<0.05)。免疫组化结果显示,DOPEY2在TMPRSS2-ERG基因融合阴性组织中的阳性表达率高于基因融合阳性组(76.5%和33.3%,P<0.05),且DOPEY2阳性的患者具有较高的Gleason分级和T分期(P<0.05)。ERG蛋白的阳性率为26.1%(12/46),且与低Gleason评分组相关,而与患者的年龄、血浆PSA值,病理T分期和远处转移等参数均无相关性。结论 DOPEY2在TMPRSS2-ERG基因融合阴性前列腺癌中的表达高于TMPRSS2-ERG基因融合阳性前列腺癌,DOPEY2可能作为一个有潜力的分子标记物指导前列腺癌分期及判断预后。Objective To study the difference and clinical significance of DOPEY2 expression between TMPRSS2-ERG gene fusion-positive and TMPRSS2-ERG gene fusion-negative prostate cancer.Methods The expressions of ERG and DOPEY2 in two prostate cancer cell lines（VCa P and PC-3） were examined by RT-PCR and Western Blot. Then, immunohistochemistry was used to detect the expression of ERG and DOPEY2 in 46 cases of prostate cancer. Results The m RNA and protein expression levels of DOPEY2 in TMPRSS2-ERG gene fusion-negative cell line（PC-3） were significantly higher than that in TMPRSS2-ERG gene fusion-positive cell line（ VCa P, P〈0. 05）. The results of immunohistochemistry showed that the expression ratio of DOPEY2 in ERG-negative and ERG-positive prostate cancer was 76.5%and 33.3% respectively（P〈0.05）. In addition, DOPEY2 was positive correlated with Gleason Grade and T stage（P〈0.05）. Over-expression ratio of ERG protein was 26.1%（12/46）, and it had significant correlation with low Gleason score（P〈0.05）, while not with age, serum PSA levels, T stage and metastasis.Conclusions There is a significant inverse correlation between the expression of ERG and DOPEY2 in both prostate cell lines and cancer tissues. DOPEY2 can serve as a helpful and prognostic biomarker in prostatic cancer.

关 键 词：前列腺肿瘤 TMPRSS2-ERG ERG DOPEY2 

分 类 号：R737.25[医药卫生—肿瘤]