Total Saponins from Dioscorea Septemloba Thunb Reduce Serum Uric Acid Levels in Rats with Hyperuricemia through OATP1A1 Up-regulation  

作　　者：陈燕 陈肖霖 项婷 孙保国 罗浩轩 刘孟婷 陈泽雄 张诗军 王昌俊 

机构地区：[1]Department of Traditional Chinese Medicine,the First Affiliated Hospital of Sun Yat-sen University [2]Department of Liver Diseases,Traditional Chinese Medical Hospital of Guangdong Province [3]Department of Traditional Chinese Medicine,Guangdong General Hospital Guangdong Academy of Medical Sciences Guangdong Geriatric Institute

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》2016年第2期237-242,共6页华中科技大学学报（医学英德文版）

基　　金：supported by grants from the National Natural Science Foundation of China(No.81072806,and No.81373500);Natural Science Foundation of Guangdong Province(No.S2012010009277);Traditional Chinese Medicine Bureau of Guangdong Province(No.20141046)

摘　　要：The aim of this study is to evaluate the efficacy of total saponins of Dioscorea（TSD）, an extract of the Chinese herbal Bi Xie, on hyperuricemia and to elucidate the underlying mechanisms. The rat hyperuricemia model was established by administration of adenine. Thirty-two rats were randomly allocated into 4 groups： model group, low/high-dose TSD-treated groups, and allopurinol-treated group. Meanwhile, 8 rats were used as normal controls. Serum uric acid（UA）, blood urea nitrogen（BUN）, serum creatinine（Scr）, and organic anion transporting polypeptide 1A1（OATP1A1） levels were measured. Comparison between the model group and treatment（allopurinol and TSD） groups showed the serum UA levels were significantly decreased in treatment groups. TSD had similar effects to allopurinol. It was found that the OATP1A1 protein expression levels in treatment groups were higher than in model group and normal controls. And different from the allopurinol-treated groups, TSD-treated group had elevated OATP1A1 expression levels in the stomach, liver, small intestine and large intestine tissues. It was suggested that TSD may facilitate the excretion of UA and lower UA levels by up-regulating OATP1A1 expression.The aim of this study is to evaluate the efficacy of total saponins of Dioscorea（TSD）, an extract of the Chinese herbal Bi Xie, on hyperuricemia and to elucidate the underlying mechanisms. The rat hyperuricemia model was established by administration of adenine. Thirty-two rats were randomly allocated into 4 groups： model group, low/high-dose TSD-treated groups, and allopurinol-treated group. Meanwhile, 8 rats were used as normal controls. Serum uric acid（UA）, blood urea nitrogen（BUN）, serum creatinine（Scr）, and organic anion transporting polypeptide 1A1（OATP1A1） levels were measured. Comparison between the model group and treatment（allopurinol and TSD） groups showed the serum UA levels were significantly decreased in treatment groups. TSD had similar effects to allopurinol. It was found that the OATP1A1 protein expression levels in treatment groups were higher than in model group and normal controls. And different from the allopurinol-treated groups, TSD-treated group had elevated OATP1A1 expression levels in the stomach, liver, small intestine and large intestine tissues. It was suggested that TSD may facilitate the excretion of UA and lower UA levels by up-regulating OATP1A1 expression.

关 键 词：Chinese herb Dioscorea septemloba Thunb DIOSCOREACEAE HYPERURICEMIA organic anion transporting polypeptide 1A1 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]