LncRNAPCGEM1和雄激素受体在前列腺癌中的共定位表达及临床意义  

Expression of lnc RNA PCGEM1 and AR co-localization in prostate cancer and tis significance

作　　者：朱竹先[1] 余晨[1] 邱忠民[2] 吕寒静[2] 关广聚[3] 张子强[2] 

机构地区：[1]同济大学附属同济医院肾内科,上海200065 [2]同济大学附属同济医院呼吸内科,上海200065 [3]山东大学第二附属医院肾内科,山东济南250014

出　　处：《中国癌症杂志》2016年第4期320-325,共6页China Oncology

基　　金：国家自然科学基金面上项目(81372513);上海市浦江人才项目(15PJ1407500)

摘　　要：背景与目的:长链非编码RNA(long non-coding RNA,lnc RNA)可参与肿瘤调控,有研究提示lnc RNA PCGEM1可能影响雄激素受体(androgen receptor,AR)通路。本研究拟检测前列腺癌lnc RNA PCGEM1和AR的表达情况,探讨其在前列腺癌中的表达及意义。方法:构建RNA核酸探针,应用荧光原位杂交(fluorescence in situ hybridization,FISH)技术检测前列腺癌lnc RNA PCGEM1的表达情况,并使用荧光免疫组织化学技术检测前列腺癌AR的表达;采用RNA-pull down技术检测PCGEM1和AR的共同作用。结果:依赖AR的LNCa P细胞的PCGEM1表达水平显著高于非AR依赖性的PC3和DU145细胞,差异有统计学意义(P<0.01)。前列腺癌组织中PCGEM1的表达与AR表达程度显著相关。与良性前列腺肿瘤相比,转移性肿瘤中的PCGEM1细胞核内定位显著不同,雄激素去势显著影响PCGEM1的表达及细胞内定位。而共定位检测发现,PCGEM1与AR之间存在一定程度的共定位现象。结论:本研究通过FISH研究发现,PCGEM1表达在雄激素去势条件下显著上调,而在转移性前列腺癌中,PCGEM1和AR的共定位表达表明,lnc RNA PCGEM1和AR相互作用可能共同参与调控前列腺癌的恶性进展及转移。Background and purpose： Long non-coding RNA （lncRNA） could be an important player in cancer biology. Recent studies showed that lncRNA PCGEMI might be important in the regulation of androgen receptor （AR） signaling pathway. We tried to observe the expressions of lncRNA PCGEM1 and AR in prostate cancer, and investigate their role and significance in prostate cancer genesis and progress. Methods： The expression of lncRNA PCGEM1 was observed in prostate cancer by fluorescence in situ hybridization （FISH） technique. Then detection of AR was performed by immunofluorescence histochemistry methods. Their co-effective role was checked by RNA pull-down technique. Results： Compared with the AR-independent cell line such as PC3 or DU145, AR-dependent cell line such as LNCaP showed much higher expression of lncRNA PCGEM1 （P〈0.01）. PCGEM1 and AR could be co-localized in most of these prostate cancer samples, especially in the metastasis samples. Moreover, androgen deprivation promoted the translocation of PCGEM1 into nucleus. RNA pull-down results also proved the co-effective role of PCGEM1 and AR. Conclusion： This study showed that lncRNA PCGEM1 was highly expressed in metastatic prostate cancer. It was related to the progress and malignant behavior of the prostate cancer. Its co-localization with AR may play an important role in prostate cancer genesis and progress.

关 键 词：长链非编码RNA 雄激素受体 前列腺癌 

分 类 号：R737.25[医药卫生—肿瘤]