GPV感染扰动雏鹅系统互作网络的研究  

Study on Perturbing Systematic Interaction Network of GPV-gosling in an Infectious Context

作　　者：朱新产[1] 王倩文[1] 朱峰伟 杨丽金[1] 

机构地区：[1]青岛农业大学生命科学学院山东省高校植物生物技术重点实验室,山东青岛266109 [2]青岛即墨畜牧兽医局,山东即墨266200

出　　处：《华北农学报》2016年第2期195-204,共10页Acta Agriculturae Boreali-Sinica

基　　金：山东省自然科学基金项目(ZR2011CM008);青岛市科技发展计划项目(12-1-4-5-(2)-jch)

摘　　要：为了解GPV(Goose parvovirus)侵染的病理学致病机理,探究GPV感染扰动雏鹅动态代谢网络平衡系统,对GPV感染雏鹅血液中的蛋白质、代谢酶活性及其同工酶结构和功能等进行生化分析。结果显示,GPV感染雏鹅的血液中,蛋白酶、Est、POD、SOD、ALP、ADH、Amy、CAT、GPT、LDH活性分别提高约41%/63%,30%/53%,50%/14%,36%/73%,156%/142%,1005%/22%,36%/26%,13%/51%,60%/244%,289%/139%;Ig G、IgM含量分别降低约50%,40%;蛋白质含量增长约50%,GPV感染雏鹅的血浆Est、SOD、ADH、Amy同工酶分别新增2,2,1,3条酶谱带,Est同工酶消减2条慢区谱带;血细胞Est、POD、SOD、Amy同工酶分别新增1,2,1,2条酶谱带;血液中CAT、LDH、GPT、ALP同工酶分别出现7,1,3,3条酶带变异,血细胞CAT、ADH同工酶分别缺少快区和慢、快区酶带,ALP同工酶在血浆与血细胞方面分别缺少慢区和快区酶带。同时显示重链59 kDa蛋白带是CK组IgM/G的共同特征,感染组Ig G还缺失258,36 kDa蛋白带;血浆与血细胞分别新增加131,86,43,33 kDa和144,104,58,53 kDa蛋白。血浆消减1条慢区酶蛋白,血细胞增加2条慢区酶蛋白。提示GPV感染应激与寄主蛋白质、蛋白酶及同工酶基因表达的协同作用,通过独特的扰动宿主动态平衡代谢网络直接或间接调控细胞易感性能。Perturbing systematic host network for Goose parvovirus（ GPV） in natural infection were still unknown. Understanding pathogenic mechanism required that protein,enzymes and isozyme from goslings infected as manifestations of network properties,rather than simply as the result of individual variations. Here blood of experimentally GPV infected goslings were examined by biochemical analysis. The results indicated that the activity of protease,Est,POD,SOD,ALP,ADH,Amy,CAT,GPT and LDH from goslings infected GPV were higher 41% /63%,30% /53%,50% /14%,36% /73%,156% /142%,1005% /22%,36% /26%,13% /51%,60% /244%,289% /139%than that of control group respectively. The content of Ig G and IgM from goslings infected GPV were lower 50%,40% than that of control group respectively,but protein was higher 50%. The isozyme of Est,SOD,ADH and Amy appeared 2,2,1,3 new enzyme band respectively,but deleted 2 slow-zone Est isozyme band in the serum of goslings infected Goose parvovirus. The isozyme of Est,POD,SOD and Amy appeared 1,2,1,2 new enzyme band in the blood corpuscle of goslings infected Goose parvovirus respectively. The isozyme of CAT,LDH,GPT and ALP appeared 7,1,3,3 variation band in the blood of goslings infected GPV respectively. The isozyme of CAT and ADH defected fast-zone and slow-zone,fast-zone band in the blood corpuscle of goslings infected Goose parvovirus respec-tively. The isozyme of ALP defected slow-zone and fast-zone band in the serum and blood corpuscle of goslings infected GPV respectively. At the same time,it also showed that the 59 kDa protein band was a common feature of IgM / G in gosling,and Ig G deleted still 258,36 kDa protein in the blood of goslings infected Goose parvovirus. The protein were increased by 131,86,43,33 kDa and 144,104,58,53 kDa band in the serum and blood corpuscle of goslings infected Goose parvovirus respectively. There were deleted 1 slow-zone zymoprotein band in the serum of goslings infected GPV,and increased 2 slow-zone zymoprotein band in the blood corpuscle of goslings infec

关 键 词：雏鹅 鹅细小病毒 蛋白/酶 同工酶 代谢网络 

分 类 号：S852.3[农业科学—基础兽医学]