一株高效铁锰氧化细菌P1的分离鉴定及氧化条件优化  

The Isolation and Identification of an Efficient Fe/Mn-oxidizing Bacterial Strain P1,and the Optimization of Its Oxidizing Conditions

作　　者：樊星[1] 王淑婷[1] 李春艳[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《生物技术通报》2016年第4期175-183,共9页Biotechnology Bulletin

基　　金："十二五"国家科技支撑计划项目(2013BAJ12B01);东北农业大学大学生SIPT计划创新训练项目(201510224257)

摘　　要：利用富集培养技术从富含铁锰的地下水井淤泥中分离得到1株能够氧化铁锰的细菌,命名为P1。经形态特征、生理生化特征和16SrDNA序列分析,将菌株P1鉴定为蜡状芽孢杆菌(Bacilluscereus)。利用单因素实验探讨菌株P1的生长及氧化特性;采用响应面分析方法考察接种量、温度、pH值3个因素对菌株P1氧化特性的影响,进一步优化菌株的氧化条件。结果表明,菌株P1的最佳氧化条件:温度28.54℃,pH7.23,接种量4.35%。在此条件下,菌株P1在锰含量为200mg/L、铁含量为800mg/L的选择性培养液中培养3d后,锰氧化率达93%以上,铁氧化率达100%。By enrichment culture, a Fe/Mn-oxidizing bacterial strain P1 was isolated from the sludge samples of groundwater well that was rich in Fe/Mn. According to morphologic and physiological-biochemical characteristics as well as 16S rDNA sequence analysis, strain P1 was identified as Bacillus cereus. Concurrently, single factor test was used to study the growth of strain P1 and its oxidation characteristics ; and the response surface methodology （ RSM ） was employed to explore the effects of inoculation size, temperature and pH on the oxidation characteristics of strain P1 and further optimize the oxidation conditions. The results showed that the optimal oxidation conditions were temperature 28.54~C, pH 7.23, and inoculation size 4.35 %. At the optimal conditions, the removal ratios of Mn and Fe were 93 % and 100 % respectively in the selective medium containing 200 mg/L Fe and 800 mg/L Mn after strain P1 was cultured for 3 d.

关 键 词：铁锰氧化 BACILLUS CEREUS 分离鉴定 氧化条件 响应面分析法 

分 类 号：Q93[生物学—微生物学]