犬细小病毒新2b型分离株的分离鉴定  

Isolation and identification of new canine parvovirus type 2b isolate

作　　者：王建科[1] 刘辉哲 林鹏[1] 赵航[1] 程悦宁[1] 张淼[1] 郭利[1] 朱洪伟[1] 武华[1] 程世鹏[1] 

机构地区：[1]中国农业科学院特产研究所吉林省特种经济动物分子生物学重点实验室,吉林长春130112 [2]河北省石家庄市卫生监督所,河北石家庄050021

出　　处：《中国兽医学报》2016年第5期734-738,共5页Chinese Journal of Veterinary Science

基　　金：吉林省自然科学基金资助项目(20140101029JC);吉林省重点科技攻关项目(20150204021NY);吉林省特种经济动物生物制品科技创新中心/中国农业科学院科技创新工程资助项目(CAAS-ASTIP-2014-ISAPS)

摘　　要：为了更好的了解我国东北地区犬细小病毒的流行情况,采用F81细胞从来自长春某宠物医院疑似患有肠炎的犬粪便样品中分离出1株病毒,经形态学、血清学、动物回归试验和分子生物学鉴定,分离的病毒为犬细小病毒new CPV-2b型,命名为CPV JL13-1。对该病毒主要结构蛋白VP2基因进行克隆测序和基因进化分析表明,CPV JL13-1分离株VP2基因与GenBank上提交的其他41株犬细小病毒株核苷酸和氨基酸均有较高的同源性,分别为98.6%-99.5%和97.6%-99.5%,其中核苷酸和氨基酸同源性最高的均为B-2004株。本研究为犬细小病毒分子流行病学调查和疫苗的研究奠定基础。To better understand the prevalence of canine parvovirus（CPV）in northeast of China,we isolated a virus from dog showing clinical and pathological signs of enteritis.This virus strain,named as CPV JL13-1,was identified as new CPV-2bfrom results of morphology,serologic test,artificial infection of Beagle dogs and molecular biology.Cloning and phylogenetic analysis of the capsid protein VP2 gene,and results indicated CPV JL13-1 VP2 gene shared higher homology with other 41 CPV isolations in GenBank.The homology of nucleotide and deduced amino acids were 98.6%-99.5% and 97.6%-99.5%,respectively.The highest degree of nucleotide homology with B-2004 strain was 99.5%,and the highest degree of deduced amino acids homology with other eight isolations,including B-2004 isolate,were 99.5%.The obtained VP2 gene sequence has been submitted to GenBank with the accession number KR058183.This study provided the basic evidence for molecular epidemiology investigation and research of vaccines of CPV.

关 键 词：犬细小病毒 新2b型 分离 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]