神经肽SP和CGRP在人慢性炎症牙髓中的表达研究  

Quantification of substance P and calcitonin gene-related peptide expressed in inflamed human dental pulp

作　　者：王伟[1,2] 于西佼[2] 杜毅[2] 

机构地区：[1]滨州医学院口腔医学院,山东烟台264003 [2]济南市口腔医院牙体牙髓科,山东济南250001

出　　处：《牙体牙髓牙周病学杂志》2016年第4期197-201,共5页Chinese Journal of Conservative Dentistry

基　　金：山东省自然科学基金(ZR2015PH017);济南市科技发展计划(201121043);济南市卫生科技发展计划(2010-32)

摘　　要：目的:观察因龋病引起的慢性牙髓炎中P物质(SP)和降钙素基因相关肽(CGRP)的表达变化。方法:选择新鲜拔除的第三磨牙45个,根据拔除前诊断分为慢性牙髓炎组、深龋组和正常组(n=15)。用免疫组化法及RT-PCR技术分别检测SP和CGRP的蛋白及基因表达变化。结果:免疫组化结果显示,慢性牙髓炎组均阳性表达SP和CGRP,且表达水平显著高于深龋组和正常组(P<0.05);RT-PCR结果显示,慢性牙髓炎组SP、CGRP mRNA的表达水平均显著高于深龋组和正常组(P<0.05),其中深龋组的SP mRNA表达水平高于正常组(P<0.05)。结论:SP和CGRP可能参与牙髓组织慢性炎症的发生。AIM： To investigate substance P（ SP） and calcitonin gene-related peptide（ CGRP） expression in dental pulp with inflammation. METHODS： Based on pulp vitality and clinical examination,45 extracted wisdom teeth were divided into chronic pulpitis,deep caries and healthy groups（ n = 15）. The expression of SP and CGRP in dental pulp tissues was detected by immunohistochemistry and RT-PCR. One-way ANOVA was used to statistically analyse the data. RESULTS： The mRNA and protein expression levels of SP and CGRP were significantly higher in the chronic pulpitis group than in deep caries group and healthy pulp group（ P 〈 0. 05）. Furthermore,SP mRNA expression was higher in deep caries group than in healthy pulp group（ P 〈 0. 05）. CONCLUSION： CGRP and SP may participate in the process of dental pulp inflammation.

关 键 词：P物质(SP) 降钙素基因相关肽(CGRP) 牙髓炎 神经肽 

分 类 号：R780.2[医药卫生—口腔医学]