基于SSR标记的荷花品种遗传多样性及群体结构分析  

Analyses on genetic diversity and population structure of lotus cultivars(Nelumbo spp.) based on SSR markers

作　　者：杜凤凤[1] 刘晓静[1] 常雅军[1] 李乃伟[1] 李丕睿[1] 姚东瑞[1] 

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014

出　　处：《植物资源与环境学报》2016年第1期9-16,共8页Journal of Plant Resources and Environment

基　　金：国家自然科学基金资助项目(31400604);江苏省自然科学基金资助项目(BK20151370);国家海洋公益性行业科研专项(201505023-4);江苏省中国科学院植物研究所基金资助项目(SQ201403)

摘　　要：采用SSR标记技术对42个荷花品种（Nelumbo spp.）的基因组DNA进行扩增,在此基础上,对供试品种进行UPGMA聚类分析、群体结构分析和主坐标分析（PCo A）。结果表明：采用17对SSR引物从42个荷花品种的基因组DNA中扩增出77个位点,多态性位点百分率为88.31%;每对引物可扩增出1~9个多态性位点。根据Nei’s遗传距离,供试的42个荷花品种可被分成Ⅰ和Ⅱ两组,分别包含3和39个品种;在Nei’s遗传距离0.150处,Ⅱ组被进一步分成Ⅱa、Ⅱb和Ⅱc 3个亚组,分别包含3、16和20个品种。群体结构分析结果表明：组分概率高于等于0.80时,供试的42个荷花品种被分成Pop1、Pop2和混合群3个亚群,分别包含17、16和9个品种。PCo A分析结果表明：在F1水平上,供试的42个荷花品种被分成2个部分;其中,Pop1亚群的品种均分布在第二和第三象限,而Pop2亚群的品种则分布在第一和第四象限。总体来看,聚类分析、群体结构分析和PCo A分析的结果基本一致。综合分析结果表明：Ⅰ组包含美洲黄莲（N.lutea Pers.）品种‘艾江南’,且与传统中国莲（N.nucifera Gaertn.）品种的亲缘关系最远,故认为该组为美洲黄莲;Ⅱ组为中国莲,其中,Ⅱc亚组以传统中国莲品种为主,而Ⅱb亚组则偏重于美洲黄莲。总体上看,供试的42个荷花品种主要被分为中国莲和美洲黄莲两组,而中美杂交莲并没有独立成组,其成因有待进一步研究。Genomic DNA of 42 lotus cultivars（ Nelumbo spp.） were amplified by SSR marker technique,on the basis,UPGMA cluster analysis,population structure analysis and principal coordinate analysis（ PCo A） of cultivars tested were carried out. The results show that 77 loci are amplified from genomic DNA of 42 lotus cultivars by 17 pairs of SSR primers,percentage of polymorphic locus is 88. 31%. Each pair of SSR primer can amplify 1- 9 polymorphic loci. According to Nei＇s genetic distance,42 lotus cultivars tested can be divided into two groups,i. e. Ⅰ and Ⅱ,which contain 3 and 39 cultivars,respectively. At Nei＇s genetic distance of 0. 150,group Ⅱ is further divided into three subgroups,i. e.Ⅱa,Ⅱ b and Ⅱ c,which contain 3,16 and 20 cultivars,respectively. The result of population structure analysis shows that when component probability is above or equal to 0. 80,42 lotus cultivars tested can be divided into three subpopulations,i. e. Pop1,Pop2 and mixed population,which contain17,16 and 9 cultivars,respectively. The result of PCo A analysis shows that on F1 level,42 lotus cultivars tested are divided into two parts. In which,cultivars of Pop1 subpopulation all distribute in the second and the third quadrants,while those of Pop2 subpopulation distribute in the first and the fourth quadrants. Overall,results of cluster analysis,population structure analysis and PCo A analysis are basically identical. The comprehensive analysis result shows that Ⅰ group contains American lotus（ N.lutea Pers.） cultivar ‘A ijiangnan ＇,and its relationship with traditional Chinese lotus（ N. nucifera Gaertn.） cultivars is the farthest,so the group is considered to be American lotus. Ⅱ group is Chinese lotus,in which,Ⅱc subgroup is mainly traditional Chinese lotus cultivars,while Ⅱb subgroup is lean to American lotus. In general,42 lotus cultivars tested are mainly divided into two groups of Chinese lotus and American lotus,while Sino-American hybrid lotus has not became into an independent group,the reas

关 键 词：荷花品种 SSR标记 遗传多样性 UPGMA聚类分析 群体结构分析 主坐标分析(PCo A) 

分 类 号：Q946-33[生物学—植物学] S682.32[农业科学—观赏园艺]