红景天多糖的提取及体外抗氧化活性研究  

Study on the Extraction of Polysaccharide from Rhodiola Rosea and Its Antioxidant Activity in Vitro

作　　者：林晓月[1,2] 齐珈娆 王丹丹[1] 任婷[1] 佟海滨[2] 孙新[2] 

机构地区：[1]北华大学药学院,吉林吉林132013 [2]吉林省分子老年医学重点实验室,北华大学生命科学中心,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2016年第3期330-334,共5页Journal of Beihua University（Natural Science）

基　　金：吉林省科技发展计划项目(20111809,20130624003JC,20140312043ZG,20150312013ZG)

摘　　要：目的优化红景天多糖的制备工艺,并系统评价红景天多糖清除自由基的能力.方法采用正交设计方法优化红景天多糖提取工艺,以多糖提率为考察指标,比较不同固液比、提取温度、提取时间、提取次数对多糖提取效果的影响;通过反复冻融、透析及酶-sevag联合脱蛋白法,纯化制备红景天多糖(RSP);采用苯酚-硫酸法、红外色谱仪及紫外-可见分光光度计对红景天多糖的基本理化性质进行表征;利用体外抗氧化模型评价红景天多糖对1,1-二苯基-2-苦苯肼自由基(DPPH·)、羟基自由基(·OH)和超氧阴离子(O_2^-·)自由基的清除能力.结果红景天多糖的最佳提取条件固液比为1∶20,提取温度为90℃,提取时间为3 h,提取次数3次.此外,红景天多糖对DPPH·和·OH具有较好的清除能力.结论成功确立红景天多糖的最佳提取工艺,红景天多糖具有一定的抗氧化作用.Objective To establish the optimum process for the extraction of Rhodiola rosea polysaccharides（RSP）,and to evaluate their free radical scavenging capacities.Method The optimum extraction conditions were obtained by orthogonal experiment design.Taking the crude polysaccharide content as index,the influences of solidliquid ratio,extraction temperature,extraction time,extraction times on the extraction efficiency were compared.The proteins in Rhodiola rosea polysaccharides were removed by repeated freezing and thawing,dialysis,and enzymesevag method,in order to acquire relatively pure polysaccharides.The basic physical and chemical properties of Rhodiola rosea polysaccharides were characterized by phenol-sulfuric acid method,infrared chromatography and UV-visible spectrophotometer.The antioxidant system was introduced in vitro.The scavenging effects of Rhodiola rosea polysaccharides on three free radicals were determined,including 1,1-diphenyl-2-picrylhydrazyl（DPPH ·）,hydroxyl radical（· OH） and superoxide anion（O_2-·）.Results The optimal extraction conditions were as follows： solid-liquid ratio of 1 ∶ 20,extraction temperature of 90 ℃,3 extraction times,extraction time of 3 h.In addition,Rhodiola rosea polysaccharides exhibited excellent scavenging abilities on DPPH· and ·OH free radicals.Conclusion Optimum extraction technology of Rhodiola rosea polysaccharide is successfully established,in addition,Rhodiola rosea polysaccharides have certain antioxidant effect.

关 键 词：红景天多糖 提取 纯化 清除自由基 

分 类 号：R28[医药卫生—中药学]