吸烟相关喉鳞状细胞癌氧化应激机制的探讨  

Study on anti-oxidase in smoking-related laryngeal squamous cell carcinoma

作　　者：刘亚超[1] 魏洁[1] 李燕萍[1] 李栋[1] 刘延斌[1] 徐国刚[1] 薛刚[1] 林彦涛[1] 尚小领[1] 

机构地区：[1]河北北方学院附属第一医院耳鼻咽喉头颈外科,河北张家口075000

出　　处：《临床耳鼻咽喉头颈外科杂志》2016年第9期733-736,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:分析吸烟相关喉鳞状细胞癌患者抗氧化酶类的表达情况,进一步探讨吸烟相关喉鳞状细胞癌的氧化应激机制。方法:连续收集2012-2015年收治的138例喉鳞状细胞癌患者和45例志愿者,根据吸烟指数和是否患病将其分为3组:A组(88例,重度吸烟喉鳞状细胞癌患者),B组(50例,不吸烟喉鳞状细胞癌患者),C组(45例,重度吸烟志愿者)。检测3组患者血清、癌组织及癌旁组织匀浆中过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)和NRF2的表达水平。结果:1A组中CAT、GSH-Px的表达水平显著低于B组(P<0.05),但高于C组(P<0.05);2A组MDA的水平显著高于B组(P<0.05)和C组(P<0.01);3喉鳞状细胞癌患者癌组织中NRF2普遍存在高表达,且其表达水平随着癌组织分化程度降低而升高(P<0.05)。结论:相比不吸烟患者,重度吸烟喉鳞状细胞癌患者将面临更加严重的氧化应激压力,喉鳞状细胞癌患者NRF2的表达水平与其病理分期有关。Objective：To investigate the role of oxidative stress in smoking-related laryngeal squamous carcinoma through detecting the expression of antioxidant enzymes in smoking patients.Method：A total of 138 cases with laryngeal squamous cell carcinoma enrolled in the first hospital affiliated the northern he bei college from 2012 to 2015and forty five volunteers were conducted.All participants were divided into three groups according to smoking index：group A（heavy smoking,88 cases of laryngeal cancer patients）and group B（no smoking 50 cases of laryngeal cancer patients）and C group（45heavy smoking volunteers）.Catalase（CAT）,glutathione peroxidase（GSH-px）and malondialdehyde（MDA）and the expression of NRF2 in serum,tissue adjacent to carcinoma,and carcinoma tissues from each groups were measured,respectively.Result：1the expression of the CAT and GSH-px in group A were significantly lower than that of group B（P〈0.05）,but higher than that of group C（P〈0.05）;2the MDA level of group A is significantly higher than group B（P〈0.05）and C group（P〈0.01）;3NRF2was highly expressed in carcinoma tissues,and the expression level was negatively correlated with degree of carcinoma differentiation（P〈0.05）.Conclusion：Compared with nonsmoking patients,heavy smoking patients with laryngeal cancer were under more severe oxidative stress.NRF2 expression level in patients with laryngeal squamous cell carcinomas was associated with pathological stage.

关 键 词：抗氧化酶 吸烟 喉鳞状细胞癌 氧化应激 

分 类 号：R739.6[医药卫生—肿瘤]