亚甲蓝晶状体前囊膜染色有效性与安全性实验研究  

Experimental research for the efficacy and cytotoxicity of methylene blue for anterior capsule staining

作　　者：刘杰为[1] 于花[1] 柴飞燕[1] 

机构地区：[1]山西省眼科医院,太原030001

出　　处：《中国实用眼科杂志》2016年第5期501-504,共4页Chinese Journal of Practical Ophthalmology

基　　金：山西省科技攻关项目（20080311066）

摘　　要：目的 研究亚甲蓝兔晶状体前囊膜染色的有效性和安全性.方法 动物实验研究.分别用0.1％、0.2％、0.5％和1.0％亚甲蓝对兔晶状体前囊膜染色,染色时间为10 s,观察亚甲蓝囊膜染色的效果,确定亚甲蓝囊膜染色的最低有效浓度,并与台盼蓝染色进行对比;并观察最低有效浓度及以上浓度的亚甲蓝染色10 s、30 s和60 s兔角膜内皮细胞形态和结构的变化.结果 0.2％及其以上浓度的亚甲蓝染色10 s、30 s和60 s均能使晶状体前囊膜有效着色.各组染色前后角膜内皮细胞形态学变化差异无统计学意义（P＞0.05）;光镜及电镜下1％和0.5％亚甲蓝60 s组角膜内皮细胞可见明显的毒性反应;两浓度30 s组有轻微毒性反应.结论 0.2％亚甲蓝染色可以使兔晶状体前囊膜有效着色,染色时间在60 s内,对兔角膜内皮细胞无明显的毒性作用.Objective To investigate the efficacy and comeal endothelial cytotoxicity of methylene blue in rabbit anterior capsule staining during cataract surgery.Methods Concentrations of 0.1%,0.2%,0.5% and 1.0% methylene blue were used to stain rabbit lens in vivo for 10 seconds.The lowest staining concentration was evaluated and all the concentration＇s staining status was compared with 0.06% trypan blue.The effect of 10 seconds and longer exposure time of 30 seconds and 60 seconds with all the concentrations on endothelial cells were also investigated.Results 0.2% and its higher concentrations of methylene blue provided comparable contrast of staining for 10 to 60 seconds of exposure.Changes in comeal endothelial cells＆#39; morphology after staining had no statistic difference at any tested concentration.But methylene blue had an obvious cytotoxicity to corneal endothelial cells at the concentration of 1% and 0.5% with the exposure time for 60 seconds under light microscope and electron microscope.A slight cytotoxicity could be tested at the two concentrations with the exposure time for 30 seconds.Conclusions 0.2% Methylene blue can be used for optimal enhancement of anterior capsule visibility for capsulorhexis,within 60 seconds of exposure time it appears to be safe as determined by no cytotoxic effects on rabbit corneal endothelial cells in vivo.

关 键 词：亚甲蓝 染色剂 前囊膜染色 连续环形撕囊 

分 类 号：R776.1[医药卫生—眼科]