双溶血环金黄色葡萄球菌的菌落特征与药物敏感性分析  

Colony features and drug susceptibility of Staphylococcus aureus with double hemolytic rings

作　　者：刘美清[1] 兰英[2] 蔡曼[2] 袁慧[1] 马旭[1] 张艳艳[1] 刘双[1] 

机构地区：[1]首都医科大学附属北京安贞医院检验科,北京100029 [2]中国科学院微生物研究所,北京100101

出　　处：《中华医院感染学杂志》2016年第9期1921-1923,共3页Chinese Journal of Nosocomiology

基　　金：教育部重点实验室开放研究基金资助项目(2013XXGB01)

摘　　要：目的通过金黄色葡萄球菌的双溶血环菌落特征及药物敏感性检测,为临床快速诊断和治疗提供依据。方法对2014年8月-2015年5月从患者送检的不同标本中分离出19株双溶血环菌株进行革兰染色镜检及血浆凝固酶试验,利用全自动微生物分析仪进行菌种鉴定和药敏试验,并对其中1株进行了16SrRNA基因序列比对分析,同时对16种常用抗菌药物进行药敏试验分析。结果双溶血环金黄色葡萄球菌菌落直径较大,内环为狭窄的β溶血,外环为宽大的α溶血,血浆凝固酶结果均呈阳性,菌种鉴定均为金黄色葡萄球菌;其中共分离出6株MRSA,检出率占31.6%;19株双溶血环金黄色葡萄球菌体外药敏试验结果显示,万古霉素、利奈唑胺、喹奴普汀/达福普汀、呋喃妥因及替加环素对金黄色葡萄球菌抗菌作用最强,敏感率均为100.0%,对利福平、莫西沙星、庆大霉素、四环素、磺胺甲噁唑/甲氧苄啶等也表现出很好的敏感性,对青霉素的耐药率最高为84.2%。结论根据金黄色葡萄球菌所具有的双溶血环的特殊菌落特征和生化试验,能够快速准确鉴定,并根据药敏试验结果合理用药,有效控制金黄色葡萄球菌的感染,以减少耐药菌株的产生和流行。OBJECTIVE To investigate the colony features and drug susceptibility of Staphylococcus aureus with double hemolytic rings so as to provide guidance for rapid clinical diagnosis and treatment.METHODS The Gram′s staining microscopy and plasma coagulase test were carried out for 19 strains of S.aureus with double hemolytic rings that were isolated from different specimens from Aug 2014 to May 2015,then the strains were identified by using automatic microorganism analyzer,the drug susceptibility testing was performed,the 16 SrRNA gene of one strain was sequenced and aligned,and the drug susceptibility rates to 16 commonly used antibiotics were analyzed.RESULTS The colony diameter of the S.aureus with double hemolytic rings was larger,the inner ring was narrow beta-haemolysis,the outer ring was wide alpha-haemolysis,all of the plasma coagulase tests showed positive,and all of the strains were identified as the S.aureus.Totally 6strains of MRSA were isolated,with the isolation rate of 31.6%.The results of in vitro drug susceptibility testing for the 19 strains of S.aureus with double hemolytic rings indicated that the drug susceptibility rates of the S.aureus strains to vancomycin,linezolid,quinupristin-dalfopristin,furantoin,and tigecycline were 100.0%;the strains showed high sensitivity to rifampicin,moxifloxacin,gentamicin,tetracycline,and sulfamethoxazole-trimethoprim;the drug resistance rate to penicillin was the highest（84.2%）.CONCLUSION The S.aureus strains can be identified rapidly and accurately according to the colony feature of double hemolytic rings and biochemical test.It is necessary to reasonably use antibiotics based on the results of drug susceptibility testing so as to effectively control the S.aureus infection and reduce the prevalence of drug-resistant strains.

关 键 词：金黄色葡萄球菌 双溶血环 16S RRNA 血浆凝固酶试验 

分 类 号：R378.11[医药卫生—病原生物学]