OCT4及BIM蛋白在卵巢浆液性肿瘤中的表达及其相关性研究  

Studying of the Expression of OCT4 and BIM Protein in Ovarian Cancer and Their Correlation

作　　者：张永琦[1] 王敏[1] 朱艳丽[1] 史聪[1] 闫效宇[1] 卓玉莲[1] 

机构地区：[1]中国医科大学附属盛京医院,110004

出　　处：《实用癌症杂志》2016年第5期723-726,共4页The Practical Journal of Cancer

摘　　要：目的研究OCT4及Bim蛋白在卵巢浆液性肿瘤中的表达及其相关性,并探讨两者在化疗耐药中的影响。方法采用免疫组化sp法,对67例卵巢浆液性囊腺癌(化疗耐药30例,敏感37例)、卵巢浆液性囊腺瘤石蜡标本10例、卵巢交界性浆液性囊腺瘤者石蜡标本10例及正常卵巢组织石蜡标本10例,进行OCT4及Bim蛋白表达情况检测。结果 OCT4蛋白阳性表达率在恶性、交界性、良性、正常组织中依次递减,差异有统计学意义(P=0.002);在耐药组与敏感组阳性表达率分别为83.33%和45.95%,差异有统计学意义(P=0.002)。Bim蛋白阳性表达率在恶性、交界性、良性、正常组织中依次递增,差异有统计学意义(P=0.002);在耐药组与敏感组阳性表达率分别为43.33%和70.27%,差异有统计学意义(P=0.026)。在化疗耐药卵巢浆液性癌中,OCT4蛋白与Bim蛋白表达无明显相关性(γ=0.03;P=0.875);在化疗敏感卵巢浆液性癌中,OCT4蛋白与Bim蛋白表达亦无明显相关性(γ=0.006;P=0.97)。OCT4蛋白与Bim蛋白表达与卵巢浆液性癌的发展呈负相关(P<0.01)。OCT4蛋白与Bim蛋白与卵巢浆液性癌临床病理因素无关。结论 OCT4及Bim蛋白与卵巢浆液性癌的发生与发展有关.OCT4蛋白与Bim蛋白表达在卵巢浆液性癌的发展呈负相关。OCT4与Bim蛋白在卵巢浆液性肿瘤的发生、发展相互作用。OCT4基因高表达,Bim基因低表达可能是造成卵巢浆液性癌对紫杉醇+铂类化疗耐药的因素之一。Objective To study the expression of the OCT4 and BIM protein in ovarian cancer and their relation,and to explore its effect on the chemotherapy drug resistance. Methods OCT4 and BIM protein expression of 67 cases of ovarian serous cystadenocarcinoma（ chemotherapy drug resistance 30 cases,sensitive 37 cases） and 10 cases of ovarian serous cystic adenoma paraffin specimens,10 cases of ovarian serous border pouch adenoma paraffin specimens and 10 cases of normal ovarian tissue paraffin specimens were det ected. Results The positive expression rate of OCT4 protein decreased progressively in ovarian serous tumors,the junction of ovarian serous tumors,benign ovarian tissues,and normal ovarian tissues,the difference was statistically significant（ P = 0. 002）; The positive expression rates of OCT4 protein in the resistant group and sensitive group were 83. 33%and 45. 95%,respectively,the difference was statistically significant（ P = 0. 002）. The positive expression rate of BIM protein increased progressively in ovarian serous tumors,the junction of ovarian serous tumors,benign ovarian tissues,and normal ovarian tissues,the difference was statistically significant（ P = 0. 002）. In the resistant group and sensitive group,the positive expression rate of BIM protein were 43. 33% and 70. 27%,respectively,the difference was statistically significant（ P = 0. 026）. In the chemotherapy drug-resistant ovarian serous carcinoma,OCT4 protein expression had no significant correlation with Bim protein expression（ γ = 0. 03; P = 0. 875）; In chemotherapy sensitive ovarian serous carcinoma,OCT4 protein expression and Bim protein expression had no obvious correlation（ γ = 0. 006; P = 0. 97）. OCT4 protein expression and Bim protein expression in ovarian serous carcinoma had negative correlation（ P〈0. 01）. OCT4 and Bim protein expression had no correlation with clinical factors. Conclusion OCT4 and BIM protein expression are associated with the occurrence and development of ovarian serous carcinoma. Bim a

关 键 词：OCT4 Bim 免疫组化 卵巢癌 化疗 耐药 敏感 

分 类 号：R737.31[医药卫生—肿瘤]