响应面法优化疏螺旋体素发酵培养基  

Optimization of fermentation medium for production of borrelidin by response surface methodology

作　　者：周剑[1] 陈宏[2] 杨煌建[1] 方东升[1] 张引[1] 张欣[1] 郑卫[1,2] 

机构地区：[1]福建省微生物研究所,福州350007 [2]福建医科大学药学院,福州350004

出　　处：《中国抗生素杂志》2016年第5期344-347,361,共5页Chinese Journal of Antibiotics

基　　金：福建省公益类科研院所省重点项目(No.2014R1009-3)

摘　　要：目的提高链霉菌BOR-0331发酵液中的生物活性成分疏螺旋体素(borrelidin)含量。方法采用单因素实验、Plackett-Burman实验、最陡爬坡试验、中心组合设计实验等方法优化发酵培养基组成及比例。结果当黄豆饼粉2.4%,甘露醇3.2%,可溶性淀粉1.8%,甘油0.7%,氯化钠0.1%时,效价最高。结论经过发酵培养基优化,效价从2.2mg/L提升至35.3mg/L,提高了1504%。Objective To enhance the productivity of borrelidin with bioactivity by Streptomyces BOR- 0331. Methods The fermentation medium were tested by single-factor experiment Plackett-Burman experiment, the steepest ascent path and Central Composite Design（CCD）. Results The productivity had enhanced from 2.2mg/L to 35.3mg/L, increasing by 1504% by using the best combination of medium with soybean meal 2.4%, mannitol 3.2%, soluble starch 1.8%, glycerol 0.7%, sodium chloride 0.1%. Conclusion The fermentation medium was optimized.

关 键 词：发酵 培养基优化 疏螺旋体素 响应面法 中心组合设计 

分 类 号：R978.1[医药卫生—药品] Q815[医药卫生—药学]