cRGD环肽修饰的脂质体靶向肺癌细胞A549的体外研究  

Study on liposomes modified by cRGD targeting to lung cancer cell A549 in vitro

作　　者：张志强[1] 马海英[2] 李运霞[1] 杨艳荣[1] 赵欣[1] 

机构地区：[1]新乡医学院第一附属医院呼吸科,河南新乡453100 [2]新乡医学院第一附属医院心外科,河南新乡453100

出　　处：《西南国防医药》2016年第5期482-485,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的构建一种整合素cRGD环肽修饰的脂质体(cRGD-LP)并用于靶向A549细胞的研究。方法采用薄膜分散法制备cRGD-LP,测定其粒径和电位,用流式细胞仪考察A549细胞对其的摄取,激光共聚焦显微镜考察cRGD-LP的肿瘤球穿透能力。结果构建的cRGD-LP粒径为(112.3±7.8)nm,粒径分散系数(PDI)为0.023±0.045,电位为(2.38±0.87)m V,且24 h内有良好的血清稳定性,粒径保持在115 nm左右。A549细胞对cRGD-LP的摄取是PEG修饰脂质体(PEG-LP)的1.7倍,且cRGD-LP对A549肿瘤球的穿透能力比PEG-LP高,能穿透至肿瘤球的深部。结论构建的cRGD-LP具有良好的靶向A549细胞的能力,是一种潜在的新型给药系统。Objective To construct a research on liposomes modified by cRGD（cRGD-LP） and its targeting ability to A549 cells in vitro. Methods The cRGD-LP was prepared by film-ultrasonic method. The size and zeta potential of cRGD-LP were detected. The cellular uptake of cRGD-LP to A549 cells was evaluated by flow cytometry. The penetration ability of cRGD-LP into the tumor spheroid was inspected by laser confocal microscopy. Results The size of cRGD-LP was around（112.3 ±7.8）nm; the polydispersity index（PDI） of cRGD-LP was 0.023±0.045; the zeta potential was（2.38±0.87）m V. The cRGD-LP was stable within 24 h and kept the particle diameter at the level of 115 nm. The cellular uptake ability of A549 to cRGD-LP was 1.7-fold as PEG-LP（liposomes modified by PEG）. The tumor spheroid penetration ability of cRGD-LP was higher than that of PEG-LP. Conclusion cRGD-LP has high tumor targeting ability to A549 cells, and it is a potential drug delivery system.

关 键 词：肺癌 整合素 cRGD 脂质体 

分 类 号：R734.2[医药卫生—肿瘤]