宫颈永生化细胞和癌细胞的核蛋白质组分析  

Differential Analysis of Nucleus Proteins of Immortalized Cervical Cell and Cervical Cancer Cell

作　　者：吕玉宇[1] 赵涌[2] 

机构地区：[1]重庆市第五人民医院病理科,重庆400062 [2]重庆医科大学病理教研室,重庆400016

出　　处：《湖北民族学院学报（医学版）》2016年第1期4-7,共4页Journal of Hubei Minzu University(Medical Edition)

摘　　要：目的应用蛋白质组学方法比较和分析人宫颈上皮永生化细胞(H8细胞株)和宫颈癌细胞(Caski细胞株)核蛋白差异表达。方法蛋白质双向凝胶电泳(2-DE)和质谱筛选H8细胞和Caski细胞核蛋白的差异表达蛋白,Western blotting验证筛选蛋白TRA16。结果成功建立H8细胞和Caski细胞核蛋白双向电泳图谱,质谱鉴定出9种差异表达的蛋白质,其中有6种(TRA16、TIAF1、CDK6、PP4C、ZN211、ANR16)在Caski中高表达,有3种(INT6、MYST1、LMNA)在H8细胞中高表达,TRA16在Caski中表达高于H8细胞(P<0.05)。结论筛选出的H8细胞和Caski细胞差异表达的核蛋白为寻找新的宫颈癌及癌前病变标记物提供帮助。Objective To compare and analyze the nucleus proteins of human endocervical cells （H8） and cervical cancer cells （Caski） by two dimensional electrophoresis（2DE） and Mass Spectrometry（ MS） .Methods The differential expression nucleus proteins of H8 ceils and Caski cells were selected by 2-DE and MS, and TRA16 was identified by Western blotting.Results Two-dimensional proein maps of H8 cells and Caski cells were obtained. 9 notable differential nucleus protein spots were detected in 2-DE gels.Among the proteins identified, 6 proteins （TRA16,TIAF1, CDK6, PP4C, ZN211, ANRld）were up-regulated in Caski cells, and 3 proteins （INT6,MYSTI,LMNA） were up-regulated in H8 cells.TRA16 express better than H8 in caski cells（P〈0.05） .Conclusion There are differentially expressed proteins in H8 cells and Caski cells which may become potential and valuable diagnostic markers for cervical cancer and CIN.

关 键 词：宫颈癌 核蛋白 蛋白组分析 TRA16 

分 类 号：R737.33[医药卫生—肿瘤]