Poly IC对急性期溃疡性结肠炎小鼠免疫机制的影响  

The effects of Poly IC on the immunologic mechanism in the acute experimental colitis

作　　者：赵红伟[1] 赵芳霞 张璟[1] 韩华[1] 岳月红[1] 

机构地区：[1]河北省人民医院,河北050051 [2]邯郸市永年县第一医院,河北057150

出　　处：《安徽卫生职业技术学院学报》2016年第2期69-70,共2页Journal of Anhui Health Vocational & Technical College

摘　　要：目的:研究Poly I:C(Polyinosinic-polycytidylic acid)对急性期溃疡性结肠炎模型小鼠免疫机制调节作用。方法:30只小鼠随机分为正常组(control)、模型组(model)、Poly I:C组(Poly I:C),建立急性溃疡性结肠炎动物模型;观察小鼠疾病活动指数(DAI)、结肠病理学变化、流式细胞术检测T淋巴细胞亚群变化、免疫比浊法检测血清免疫球蛋白变化、免疫组织化学染色测定结肠组织ICAM-1变化;结果:经Poly I:C治疗后模型小鼠的疾病活动指数及病理组织评分明显下降;与model组比较,Poly I:C组免疫细胞反应明显减轻弱,结肠组织ICAM-1蛋白表达明显降低。结论:可见Poly I:C对急性溃疡性结肠炎模型小鼠有免疫调节作用。Objective：To investigate the effects of Poly IC on the immunologic mechanism in DSS-induced acute colitis.Method：Thirty of mice were randomly grouped as follows： control group, model group, Poly IC group, acute colitis were established, disease activity index （DAI） and colon pathological changes were observed,the changes of T lymphocyte subsets and immunoglobulin were detected by Flow cytometry and Immune turbidimetry, the expressions of ICAM-1 proteins were detected by immunohistoehemistry,Results：The DAI and colon pathological were changed in the Poly hC group compared with that of the model group; Immunologic cells were decreased, the proteins expressions of ICAM-1 significantly decreased in the Poly I：C group compared with that of the model group by immunohistochemical staining.Conclusions：The administration of PolyI：C may be adjusted the immunologic mechanism in the acute experimental colitis.

关 键 词：Voly I:C 溃疡性结肠炎 急性动物模型 免疫细胞 免疫机制 

分 类 号：R574.62[医药卫生—消化系统]