亚低温对急性脑梗死并脑心综合征大鼠血清一氧化氮水平的影响及意义  

Influence of mild hypothermia on serum nitric oxide level and its significance in acute cerebral infarction rats with cerebral cardiac syndrome

作　　者：仇学亮 陈涛[2] 杜业亮[2] 孙阿林 谢地成 张国强[1] 

机构地区：[1]潍坊医学院神经病学教研室,山东潍坊261042 [2]潍坊市人民医院脑科医院神经内科,山东潍坊261021

出　　处：《中华实用诊断与治疗杂志》2016年第5期449-451,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：潍坊市科技发展计划(2014WS082)

摘　　要：目的探讨亚低温对急性脑梗死并脑心综合征大鼠血清一氧化氮水平的影响及其临床意义。方法 Wistar大鼠95只,连续监测Ⅱ导联标准心电图15min,选取无心电图异常大鼠90只,随机分为亚低温组、常温组和假手术组,每组30只。亚低温组和常温组采用线栓法栓塞大鼠右侧大脑中动脉;假手术组仅分离颈总动脉、颈外动脉、颈内动脉,但不结扎及插入线栓。术后低温组维持亚低温状态2h,余2组维持正常体温。术后连续监测标准Ⅱ导联心电图2h。记录各组脑心综合征发生情况,采用ELISA法检测各组术后2、24、48h血清一氧化氮水平,并进行比较。结果假手术组均未发生脑心综合征,亚低温组脑心综合征发病率(46.7%)低于常温组(76.7%)(P<0.05);术后24、48h时常温组[(57.93±10.38)、(76.78±7.14)μmol/L]和亚低温组一氧化氮水平[(57.57±9.38)、(64.37±6.54)μmol/L]均明显高于假手术组[(51.31±11.22)、(53.02±8.65)μmol/L](P<0.05);术后2h常温组[(53.90±9.40)μmol/L]和亚低温组一氧化氮水平[(53.02±7.83)μmol/L]高于假手术组[(48.87±7.46)μmol/L],但差异无统计学意义(P>0.05);常温组术后48h时一氧化氮水平高于亚低温组(P<0.01),术后2、24h高于亚低温组,但差异无统计学意义(P>0.05)。结论亚低温可降低急性脑梗死大鼠脑心综合征发生率,其可能机制是抑制病理状态下一氧化氮水平的过度增高。Objective To explore the influence of mild hypothermia on serum nitric oxide （NO） level and its significance in acute cerebral infarction rats with cerebral cardiac syndrome （CCS）. Methods Ninety-five Wistar rats were continuously monitored Ⅱ-lead standard ECG for 15 minutes, in which 90 rats with normal ECG were selected and randomly divided into mild hypothermia group, normal temperature group and sham-operation group, with 30 rats in each group. Right middle cerebral artery of the rats in mild hypothermia group and normal temperature group were occluded with nylon thread and the carotid artery, external carotid artery and internal carotid artery were isolated but not ligated or inserted thread plug in sham group. All rats received Ⅱ-lead ECG monitor continuously for 2 hours. Mild hypothermia group remained mild hypothermia and the other two groups remained normal temperature. The incidences of CCS were recorded. The serum NO levels were measured with ELISA in 2, 24 and 48 hours after operation and compared among groups. Results No CCS was found in sham-operation group. The incidence of CCS was significantly lower in mild hypothermia group （46.7%） than that in normal temperature group （76.7%） （P〈0.05）. The NO levels in 24 and 48 hours after operation were significantly higher in normal temperature group （（57.93 ± 10.38）, （76.78±7.14） μmol/L） and mild hypothermia group （（57.57±9.38）, （64.37±6.54） μmol/L） than those in sham-operation group （（51.31±11.22）, （53.02±8. 65） μmol/L） （P〈0.05）. And the NO level in 2 hours after operation was higher in normal temperature group （（53. 90 ± 9. 40） ptmol/L） and mild hypothermia group （（53. 02 ± 7. 83）μmol/L） than that in sham-operation group （（48.87±7.46）μmol/L）, but there was no significant difference （P〉0.05）. The NO level in 48 hours after operation was significantly higher in normal temperature group than that in mild hypothermia group （P〈0.01）, and NO lev

关 键 词：急性脑梗死 脑心综合征 一氧化氮 亚低温 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R541[医药卫生—临床医学]