两个品种马骨髓基因表达谱分析  

Analysis of Gene Expression Profile of Bone Marrow in Two Horse Breeds

作　　者：赵一萍[1] 张宇宏[1] 李蓓[1] 黄金龙[1] 白东义[1] 赵启南[1] 乌尼尔夫[1] 杨丽华[1] 吴敬[1] 乌云达来[1] 芒来[1] 

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古自治区蒙古马遗传资源保护及马产业工程实验室,呼和浩特010018

出　　处：《畜牧兽医学报》2016年第5期922-930,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：内蒙古自治区科技厅重点实验室建设项目(20130902);内蒙古自治区科技厅应用技术研究与开发项目(20140172);内蒙古自治区自然科学基金项目(2015BS0316)

摘　　要：为了寻找不同品种马骨髓之间的差异表达基因,本研究构建了蒙古马-骨髓和纯血马-骨髓基因表达谱文库,经Illumina Miseq高通量测序,分别获得了7 219 956和7 116 170个有效reads;两个文库映射到11、8和1号染色体的reads最多,而映射到29、30和31号染色体的reads最少;两个文库共筛出318个差异表达基因,其中与免疫相关的基因有21个。GO功能富集分析结果表明差异表达基因与转运、细胞间信号传导、生物学过程和细胞质等有关;KEGG通路富集分析结果表明,差异最大的显著富集KEGG条目为神经系统。这些结果为研究马匹抗病力的遗传和免疫机理等提供基础数据。To investigate the differential genes expression of bone marrow between different horse breeds,expression profiling libraries of Mongolian Horse-bone marrow and Thoroughbred-bone marrow were constructed. We obtained 7 219 956 and 7 116 170 useful reads by the Illumina Miseq sequencing, respectively. The most of reads in the 2 expression profiling libraries were maped on chromosome 11, chromosome 8 and chromosome 1, and the least of reads were maped on chromosome 29, chromosome 30 and chromosome 31. 318 differentially expressed genes were found in 2 expression profiling libraries,including 21 immune-related genes. The Gene Ontology analysis showed that the differentially expressed genes were related to transport, cell-cell signa- ling, biological process and cytoplasm, etc. The Kyoto Encyclopedia of Genes and Genomes analy- sis indicated that nervous system was the most differentiated KEGG term. These results will pro- vide data basis for further studies on the horses disease resistance and immune mechanism.

关 键 词：马 骨髓 表达谱 差异基因 

分 类 号：S821.2[农业科学—畜牧学]